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Transcriptome of ZmPMP3g overexpressing maize inbred line Ye478, and wild type Ye478 under control, moderate drought, exogenous abscisic acid, or treatment combinations

ABSTRACT: This study is to identify the role of maize ZmPMP3g (GenBank accession no. EC869579.1), a proteolipid membrane potential modulator 3 (PMP3) domain gene, in drought tolerance of maize, and the genes and signaling pathways under over-expression of ZmPMP3g

ORGANISM(S): Zea mays

PROVIDER: GSE220124 | GEO | 2022/12/11

REPOSITORIES: GEO

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Transcriptome of ZmPMP3g overexpressing maize inbred line Ye478, and wild type Ye478 under control, moderate drought, exogenous abscisic acid, or treatment combinations

Project description:Transcriptome of ZmPMP3g overexpressing maize inbred line Ye478, and wild type Ye478 under control, moderate drought, exogenous abscisic acid, or treatment combinations

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Exogenous abscisic acid induces the lipid and flavonoid metabolism of tea plants under drought stress.

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Exogenous abscisic acid significantly affects proteome in tea plant (Camellia sinensis) exposed to drought stress.

Project description:Tea [Camellia sinensis (L.) O. Kuntze] is an important economic crop, and drought is the most important abiotic stress affecting yield and quality. Abscisic acid (ABA) is an important phytohormone responsible for activating drought resistance. Increased understanding of ABA effects on tea plant under drought stress is essential to develop drought-tolerant tea genotypes, along with crop management practices that can mitigate drought stress. The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress. Leaf protein patterns of tea plants under simulated drought stress [(polyethylene glycol (PEG)-treated] and exogenous ABA treatment were analyzed in a time-course experiment using two-dimensional electrophoresis (2-DE), followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Among the 72 protein spots identified by MALDI-TOF MS, 16 proteins were downregulated and two were upregulated by exogenous ABA. The upregulated proteins have roles in glycolysis and photosystem II stabilization. Twenty-one protein spots were responsive to drought stress and most participate in carbohydrate and nitrogen metabolism, control of reactive oxygen species (ROS), defense, signaling or nucleic acid metabolism. The combined treatments of exogenous ABA and drought showed upregulation of 10 protein spots at 12 h and upregulation of 11 proteins at 72 h after initiation of drought stress. The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

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Project description:To understand the transcriptome changes during drought tolerance in maize, the drought-tolerant line Han21 and drought-sensitive line Ye478, which show substantial differences in drought tolerance at the seedling stage, were selected for this study. Using the GeneChip Maize Genome Arrays, we applied genome-wide gene expression analysis to the two genotypes under gradual drought stress and re-watering. We identified 2172 common regulated transcripts in both lines under drought stress, with 1084 common up-regulated transcripts and 1088 common down-regulated transcripts. Among the 2172 transcripts, 58 potential protein kinases and 117 potential transcription factors were identified. The potential components of the ABA signaling pathway were identified from the common regulated transcripts. We also identified 940 differentially regulated transcripts between the two lines. Among the 940 transcripts, the differential expression levels of 29 transporters and 15 cell wall-related transcripts may contribute to the different tolerances of the two lines. Additionally, we found that the drought-responsive genes in the tolerant Han21 line recovered more quickly when the seedlings were re-watered, and 311 transcripts in the tolerant Han21 line were exclusively up-regulated at the re-watering stage compared to the control and stress conditions. Our study provides a global characterization of two maize inbred lines during drought stress and re-watering and will be valuable for further study of the molecular mechanisms of drought tolerance in maize.

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SelT knockdown mouse fibroblast cells vs control cells

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Genome-wide transcriptome analysis of two maize inbred lines under drought stress during the seedling stage

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SelT knockdown mouse fibroblast cells vs control cells

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