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Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [16S]

ABSTRACT: To determine how constitutively active autophagy in Becn1F121A mice affects the fecal microbiota, we performed a 16s analysis.

ORGANISM(S): synthetic construct mouse gut metagenome

PROVIDER: GSE220883 | GEO | 2023/02/03

REPOSITORIES: GEO

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Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [RNA-seq]

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Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress.

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Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress super

Project description:This SuperSeries is composed of the SubSeries listed below.

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Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [16S]

Project description:Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [16S]

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Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [RNA-seq]

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16S-seq analysis of the microbiota of WT and TCRb-/- mice

Project description:To examine the microbiota abundance difference, we performed fecal 16s sequencing of wild type, TCRb-/-, TCRb-/- co-housed with WT and TCRb-/- receiving WT T cells.

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Cathelicidin deficiency disrupts goblet cell mucus secretion but not mucus associated proteins in Citrobacter rodentium-induced colitis

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NLRP6 inflammasome orchestrates the colonic host-microbial interface by regulating goblet cell mucus secretion.

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Project description:Background & aimsInfections resulting from intestinal yeast and bacteria affect a large number of patients with deficits in absorptive or secretory epithelial transport mechanisms. The basolateral Na+-K+-2Cl- cotransporter (NKCC1) has been implicated in intestinal epithelial fluid secretion. Two patients with deleterious heterozygous (NKCC1-DFX, DFX for Asp-Phe-stop codon) or homozygous (Kilquist) mutations in SLC12A2 (NKCC1) suffered from gastrointestinal deficits. Because of chronic infections, the colon and the small intestine of the NKCC1-DFX patient were resected surgically.MethodsTo investigate how NKCC1 affects the integrity and function of the gut epithelia, we used a mouse model recapitulating the NKCC1-DFX patient mutation. Electron microscopy and immunostaining were used to analyze the integrity of the colonic mucus layers and immune cell infiltration. Fluorescence in situ hybridization was performed on the distal colon sections to measure bacteria translocation to the mucosa and submucosa. Citrobacter rodentium was used to measure mouse ability to clear enteric infection. A multiplex cytokine assay was used to analyze mouse inflammatory response to infection.ResultsWe show that NKCC1-DFX expression causes defective goblet cell mucus granule exocytosis, leading to secretion of intact granules into the lumen of the large intestine. In addition, NKCC1-DFX colon submucosal glands secrete mucus that remained attached to the epithelium. Importantly, expression of the mutant NKCC1 or complete loss of NKCC1 function leads to aggravated inflammatory response to C rodentium infection. Compared with wild-type, NKCC1-DFX mice showed decreased expression of claudin-2, a tight junction protein involved in paracellular Na+ and water transport and enteric infection clearance.ConclusionsOur data indicate that NKCC1-DFX impairs gut barrier function by affecting mucus secretion and immune properties.

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