Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [16S]

ABSTRACT: To determine how constitutively active autophagy in Becn1F121A mice affects the fecal microbiota, we performed a 16s analysis.

ORGANISM(S): synthetic construct mouse gut metagenome

PROVIDER: GSE220883 | GEO | 2023/02/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress.

Project description:Colonic goblet cells are specialized epithelial cells that secrete mucus to physically separate the host and its microbiota, thus preventing bacterial invasion and inflammation. How goblet cells control the amount of mucus they secrete is unclear. We found that constitutive activation of autophagy in mice via Beclin 1 enables the production of a thicker and less penetrable mucus layer by reducing endoplasmic reticulum (ER) stress. Accordingly, genetically inhibiting Beclin 1-induced autophagy impairs mucus secretion, while pharmacologically alleviating ER stress results in excessive mucus production. This ER-stress-mediated regulation of mucus secretion is microbiota dependent and requires the Crohn's-disease-risk gene Nod2. Overproduction of mucus alters the gut microbiome, specifically expanding mucus-utilizing bacteria, such as Akkermansia muciniphila, and protects against chemical and microbial-driven intestinal inflammation. Thus, ER stress is a cell-intrinsic switch that limits mucus secretion, whereas autophagy maintains intestinal homeostasis by relieving ER stress.

| S-EPMC10016318 | biostudies-literature

Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress super

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-03 | GSE220884 | GEO

Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [RNA-seq]

Project description:To determine how constitutively active autophagy in Becn1F121A mice affects the colonic tissue, we performed a transcriptional analysis.

2023-02-03 | GSE220457 | GEO

Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [16S]

Project description:Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [16S]

| PRJNA911803 | ENA

16S-seq analysis of the microbiota of WT and TCRb-/- mice

Project description:To examine the microbiota abundance difference, we performed fecal 16s sequencing of wild type, TCRb-/-, TCRb-/- co-housed with WT and TCRb-/- receiving WT T cells.

2024-05-06 | GSE220867 | GEO

Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress super

Project description:Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress super

| PRJNA911802 | ENA

Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [RNA-seq]

Project description:Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress [RNA-seq]

| PRJNA909805 | ENA

Impact of fecal microbiota transplantation (FMT) in 3 days-ABX-treated recipient mice using feces from 6 NSCLC patients at diagnosis.

Project description:This study aimed to analyze changes in gut microbiota composition in mice after transplantation of fecal microbiota (FMT, N = 6) from the feces of NSCLC patients by analyzing fecal content using 16S rRNA sequencing, 10 days after transplantation. Specific-pathogen-free (SPF) mice were used for each experiments (N=4) as controls.

2023-07-01 | E-MTAB-12812 | biostudies-arrayexpress

Ascending colon-derived mucin-type O-glycans form key mucus layers encapsulating microbiota in the colon (16S rNA-seq)

Project description:The colon contains a dense metabolically potent microbiota. The colonic O-glycan-rich mucus has been recognized as a key barrier to prevent microbial intrusion, but how this system forms and functions remains unclear. Here, we discovered that the colon mucus is mainly forged by microbiota-dependent secretion of O-glycosylated Muc2 by goblet cells in the ascending colon, where it seamlessly encapsulates the fecal materials including the microbiota. Deletion of O-glycans in the ascending colon impaired the segregating function of the mucous coating, leading to altered structure and metabolic output of the microbiota, and transcriptional homeostasis of the entire host mucosa. These findings represent a paradigm change of the prevailing model of the colon mucus system and provides new insights into host and microbiota symbiosis.

2020-10-28 | GSE133256 | GEO

16S Analysis of Poly I:C treated mouse to induce ASD

Project description:Fecal Microbiota rawdata from autistic model mice. Female and Male Poly I:C treated mice were compared at microbiota level.

2023-05-04 | E-MTAB-11150 | biostudies-arrayexpress