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Single-cell RNA-sequencing (scRNA-seq) of GFP+cells isolated from Tg(Brn3c:mGFP) 6 days zebrafish with GFP expression in all hair cells.

ABSTRACT: Hair cells play key roles in hearing and balance, and hair cell loss would result in hearing loss or vestibular dysfunction. Zebrafsh, owing to their hair cell-enriched organs, have been widely applied in hair cell-related research worldwide. In this study, we analyzed the GFP+cells isolated from Tg(Brn3c:mGFP) larvae with GFP expression in all hair cells using single-cell RNA-sequencing (scRNA-seq). Three subtypes of hair cells, namely macula hair cell (MHC), crista hair cell (CHC), and neuromast hair cell (NHC), were characterized and validated by whole-mount in situ hybridization analysis of marker genes. The hair cell scRNA-seq data revealed hair cell-specifc genes, including hearing loss genes that have been identifed in humans and novel genes potentially involved in hair cell formation and function.

ORGANISM(S): Danio rerio

PROVIDER: GSE221471 | GEO | 2023/01/03

REPOSITORIES: GEO

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