Transcriptomics

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Identification of G-quadruplex-interacting Proteins in Living Cells using an artificial G4-targeting Biotin ligase


ABSTRACT: We engineering a G4-targeted biotin ligase probe (G4PID), which consists of three parts, RHAU23 at the C-terminus, miniTurbo at the N-terminus, and a flexible linker in the middle to prevent the disturbance of the two functional moieties. We used eCLIP to verified the G4s affinity of G4PID. We also used eCLIP method to verify the direct G4 interaction of Splicing factor 3B subunit 4 (SF3B4) in cellular transcriptome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE223574 | GEO | 2024/03/07

REPOSITORIES: GEO

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