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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [CUT&Tag]

ABSTRACT: We find that two SAM synthases in C. elegans, SAMS-1 and SAMS-3/4, contribute differently to modification of H3K4me3, gene expression and survival in the heat stress response. Our data showing differential SAM provisioning to distinct groups of methylated targets based on SAM synthase source suggests that determining how methyl donors are provided will broaden insight into 1CC functions in aging and stress.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE223594 | GEO | 2023/02/13

REPOSITORIES: GEO

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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams RNAi, 20C]

Project description:We find that two SAM synthases in C. elegans, SAMS-1 and SAMS-3/4, contribute differently to modification of H3K4me3, gene expression and survival in the heat stress response. Our data showing differential SAM provisioning to distinct groups of methylated targets based on SAM synthase source suggests that determining how methyl donors are provided will broaden insight into 1CC functions in aging and stress.

2023-02-13 | GSE223595 | GEO

S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams-4 RNAi, Heat shock]

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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams RNAi, 20C]

Project description:S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams RNAi, 20C]

| PRJNA926942 | ENA

S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress

Project description:S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress

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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams-4 RNAi, Heat shock]

Project description:S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [RNA-seq: sams-4 RNAi, Heat shock]

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S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-13 | GSE223597 | GEO

S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [CUT&Tag]

Project description:S-adenosylmethionine synthases specify distinct H3K3me3 populations and gene expression patterns during heat stress [CUT&Tag]

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Sympathetic neuron-associated macrophages contribute to obesity by importing and metabolizing norepinephrine

Project description:The cellular mechanism(s) linking macrophages to norepinephrine (NE)-mediated regulation of thermogenesis has been a topic of debate. Here, we identify sympathetic neuron-associated macrophages (SAMs) as a population of macrophages that mediate clearance of NE via expression of Slc6a2, an NE transporter, and monoamine oxidase A (MAOa), a degradation enzyme. Optogenetic activation of the SNS upregulates NE uptake by SAMs and shifts the SAM profile to a more pro-inflammatory state. NE uptake by SAMs is prevented by genetic depletion of Slc6a2 or inhibition of the transporter. We also found that obesity increases SAM content in the SNS. In two mouse models of obesity, genetic ablation of Slc6a2 in SAMs increases brown adipose tissue (BAT) content, causes browning of white fat, increases thermogenesis and leads to significant and sustained weight loss. We further show that this pathway is conserved as human sympathetic ganglia also contain SAMs and the analogous molecular machinery for NE clearance, thus constituting a potential target for obesity treatment.

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Mechanisms of Myelocytic Nitric Oxide Synthases Against Hypoxia-Induced Pulmonary Hypertension

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Project description:We evaluated a new miRNA profiling platform that utilizes Illuminaâs existing robust DASL chemistry as the basis for the assay. Using total RNA from five colon cancer patients and four cell lines, we evaluated the reproducibility of miRNA expression levels across replicates and with varying amounts of input RNA. The beta test version was comprised of 735 miRNA targets of Illuminaâs miRNA profiling application. The study used four SAMs to assess variability due to separate total RNA extractions, technical replicates of each extraction and varying total RNA input. Total RNA extracted from five patient samples (IDs 45, 165, 565, 919, 133) and RNA from four cell lines (HeLa, PC3, 293 and MCF-7) supplied by Illumina, was evaluated on each of 4 SAMs. SAM 1 was hybridized in week one, SAMs 2 and 3 were hybridized in week two and SAM 4 (with cell line RNA only) was hybridized in week three of the study. Sample allocation on SAM 1 was designed to assess reproducibility between two separate total RNA extractions, between duplicate samples (technical replicates) and between two input RNA amounts. Sample allocation on SAM 2 was designed to assess technical reproducibility and the effect of six input levels of total RNA. Sample allocation on SAM 3 was designed to assess technical replication. The four cell lines, but none of the 5 patient RNA, were run on a fourth SAM . Reproducibility across all four SAMs was also assessed.

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