Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mid2 mutant 3 hour exposure to zymolyase

ABSTRACT: We did transcription profiling on the effect of mid2 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE22407 | GEO | 2010/06/19

SECONDARY ACCESSION(S): PRJNA129071

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

hog1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of hog1 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response

2008-09-06 | GSE9931 | GEO

wsc1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of wsc1 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response

2010-06-19 | GSE22403 | GEO

sho1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of sho1 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response

2010-06-19 | GSE22408 | GEO

rlm1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of rlm1 deletion, gene involved in cell wall stress response Keywords: cell wall stress response

2008-09-06 | GSE9933 | GEO

slt2 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of slt2 deletion, gene involved in cell wall stress response Keywords: cell wall stress response

2008-09-06 | GSE9932 | GEO

hog1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of hog1 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response S. cerevisiae (hog1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 Â°C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.

2010-06-19 | E-GEOD-9931 | biostudies-arrayexpress

msn2/4 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of msn2 msn4 deletion, genes involved in several stress process Keywords: cell wall stress response

2008-09-06 | GSE9934 | GEO

rlm1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of rlm1 deletion, gene involved in cell wall stress response Keywords: cell wall stress response S. cerevisiae (rlm1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 Â°C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.

2010-06-19 | E-GEOD-9933 | biostudies-arrayexpress

slt2 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of slt2 deletion, gene involved in cell wall stress response Keywords: cell wall stress response S. cerevisiae (slt2 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 Â°C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.

2010-06-19 | E-GEOD-9932 | biostudies-arrayexpress

mid2 mutant 2 hour exposure to caspofungin

Project description:We did transcription profiling on the effect of wsc1 deletion, gene involved in cell wall stress response Keywords: cell wall stress response

2010-06-19 | GSE22414 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data