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Hog1 mutant 3 hour exposure to zymolyase

ABSTRACT: We did transcription profiling on the effect of hog1 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE9931 | GEO | 2008/09/06

SECONDARY ACCESSION(S): PRJNA114685

REPOSITORIES: GEO

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hog1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of hog1 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response S. cerevisiae (hog1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 Â°C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.

2010-06-19 | E-GEOD-9931 | biostudies-arrayexpress

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rlm1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of rlm1 deletion, gene involved in cell wall stress response Keywords: cell wall stress response

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Project description:We did transcription profiling on the effect of slt2 deletion, gene involved in cell wall stress response Keywords: cell wall stress response

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Wild type 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of Zymolyase in Saccharomyces cerevisiae using strains BY4741 (wild type). Yeast cells exposed to Zymolyase in YPD growth medium show a significant induction of cell wall compensatory mechanism. Keywords: cell wall stress response

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msn2/4 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of msn2 msn4 deletion, genes involved in several stress process Keywords: cell wall stress response

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rlm1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of rlm1 deletion, gene involved in cell wall stress response Keywords: cell wall stress response S. cerevisiae (rlm1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 Â°C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.

2010-06-19 | E-GEOD-9933 | biostudies-arrayexpress

slt2 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of slt2 deletion, gene involved in cell wall stress response Keywords: cell wall stress response S. cerevisiae (slt2 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 Â°C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.

2010-06-19 | E-GEOD-9932 | biostudies-arrayexpress

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