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EVG1 regulates vascular development and regeneration in response to stress

ABSTRACT: Vascular tissues possess remarkable regenerative abilities, quickly healing post damage from wounding, grafting, or drought. The regenerative ability is also relevant for many plant pathogens that invade host tissues and induce the formation of vasculature to derive nutrients, thereby suggesting a possible mechanistic overlap in vascular regeneration between stresses. With comparative transcriptomics we identified a gene that was highly upregulated in Agrobacterium infection, nematode infection, and during graft formation. Loss of function mutant of EVG1 enhanced ectopic xylem formation in Vascular cell Induction culture System Using Arabidopsis Leaves (VISUAL) and enhanced graft formation and was termed ENHANCER OF VISUAL AND GRAFTING 1 (EVG1). EVG1 promoted cambium development and inhibited vascular regeneration during grafting. Transcriptomic analyses found that stress responsive, cell wall related, and ABA response related genes were differentially expressed in evg1. Mutants of EVG1 also phenocopied mutants of RLP44, a receptor that interacts with the receptors of both the brassinosteroid (BR) and phytosulfokine (PSK) signaling pathway to incorporate cell wall damages cues into hormone signaling and help maintain procambium cell identity. We identified that EVG1 is highly stress responsive while RLP44 is not despite showing similar phenotypes in developmental assays. We propose that EVG1 and RLP44 act in the same pathway. EVG1 might act as a stress signal and regulates development while indirectly also influencing the RLP44 associated pathways.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE224565 | GEO | 2025/01/09

REPOSITORIES: GEO

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