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Transcriptional profiling of Arabidopsis shoots and roots responses to phytosulfokine (PSK)

ABSTRACT: Phytosulfokine (PSK) is a plant peptide hormone that contributes to plant signaling and induced various of effects, including growth, senescence, stress tolerant and defense responses. PSK is a pentapeptide with two tyrosine sulfated by tyrosylprotein sulfotransferase (TPST) enzyme. The sulfated PSK can bind to its cell surface receptors, but the transcriptional readouts remain largely unknown. Here, we treated tpst mutant that are unable to produce native sulfated PSK with synthetic active PSK to capture time-series and tissue-specific readouts of PSK signaling.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE254986 | GEO | 2024/10/17

REPOSITORIES: GEO

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Transcriptional profiling of Arabidopsis seedlings responses to phytosulfokine (PSK)

Project description:Phytosulfokine (PSK) is a plant peptide hormone that contributes to plant signaling and induced various of effects, including growth, senescence, stress tolerant and defense responses. PSK is a pentapeptide with two tyrosine sulfated by tyrosylprotein sulfotransferase (TPST) enzyme. The sulfated PSK can bind to its cell surface receptors, but the transcriptional readouts remain largely unknown. Here, we treated tpst mutant that are unable to produce native sulfated PSK with synthetic active PSK to capture concentration-sensitive readouts of PSK signaling.

2024-10-17 | GSE254987 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of PSKγ1ox and control Transcriptomes

Project description:Phytosulfokine-α (PSK-α), a sulfated pentapeptide hormone with the sequence YIYTQ, plays important roles in many aspects of plant growth and development. In this study, we identified a pair of putative precursor genes in soybean, GmPSKγ1 and -2, encoding a PSK-like peptide: PSK-γ. Similar to PSK-α in amino acid composition, the sequence of PSK-γ is YVYTQ, and the tyrosines undergo sulfonylation. Treatment of Arabidopsis seedlings with synthetic sulfated PSK-γ significantly enhanced root elongation, indicating that PSK-γ might be a functional analog of PSK-α. Expression pattern analysis revealed that the two GmPSKγ genes, especially GmPSKγ1, are primarily expressed in developing soybean seeds. Heterologous expression of GmPSKγ1 under the control of a seed-specific promoter markedly increased seed size and weight in Arabidopsis, and this promoting effect of PSK-γ on seed growth was further confirmed in transgenic tobacco constitutively expressing GmPSKγ1. Cytological analysis of transgenic Arabidopsis seeds revealed that PSK-γ promotes seed growth by inducing embryo cell expansion. In addition, transcriptome analysis of GmPSKγ1-expressing Arabidopsis seeds suggested that PSK-γ signaling may regulate cell wall loosening to promote cell expansion. Overall, our results shed light on the mechanism by which PSK-γ promotes seed growth, paving the way for the use of this new peptide for biotechnological improvement of crop seed/grain size and yield.

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A role of PSK-CPK28-GS2 signalling in optimizing growth and defence in tomato

Project description:Phytosulfokine (PSK) is a plant pentapeptide hormone that fulfills a wide range of functions. Although it has been frequently reported to function in the inverse regulation of growth and defence in response to (hemi)biotrophic pathogens, the mechanism involved remains largely unknown. Using tomato (Solanum lycopersicum) and Pseudomonas syringae pv. tomato (Pst) DC3000 pathogen as a system, we present compelling evidence that the tomato PSK receptor PSKR1 interacts with the calcium-dependent protein kinase CPK28, which phosphorylates the key enzyme of nitrogen assimilation glutamine synthetase GS2 at two sites (S334 and S360 sites). These post translational modifications uncouple the two PSK-induced effects on defence responses and on growth regulation, respectively. The discovery of these sites will inform breeding strategies designed to optimize the growth-defence balance. The concept that such peptide hormones can regulate plant growth and resistance in a compatible manner is attractive, not least because crop yields and fitness can be optimized simultaneously in global climate-change era.

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Transcriptional profiling of Arabidopsis shoots and roots responses to phytosulfokine (PSK)

Project description:Transcriptional profiling of Arabidopsis shoots and roots responses to phytosulfokine (PSK)

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Transcriptional profiling of Arabidopsis responses to phytosulfokine (PSK)

Project description:Our study reveals that phytosulfokine (PSK) promotes plant growth by suppressing defense mechanisms, highlighting a key link between growth regulation and immune response. By demonstrating how PSK downregulates WRKY TFs and associated kinase activity, this research provides valuable insights into plant biology, offering potential strategies for improving crop yield and pathogen resistance. This SuperSeries is composed of the SubSeries listed below.

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Transcriptional profiling of Arabidopsis responses to phytosulfokine (PSK)

Project description:Transcriptional profiling of Arabidopsis responses to phytosulfokine (PSK)

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Transcriptional profiling of Arabidopsis seedlings responses to phytosulfokine (PSK)

Project description:Transcriptional profiling of Arabidopsis seedlings responses to phytosulfokine (PSK)

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Gene expression in roots and shoots of plants grown on selenate

Project description:Selenate is chemically similar to sulfate and can be taken up and assimilated by plants via the same transporters and enzymes. In contrast to many other organisms, selenium (Se) has not been shown to be essential for higher plants. In excess, Se is toxic and restricts development. Both Se deficiency and toxicity pose problems worldwide. To obtain better insight into the effects of Se on plant metabolism and into plant mechanisms involved in Se tolerance, the transcriptome of Arabidopsis plants grown with or without selenate was studied, and Se-responsive genes identified. Roots and shoots exhibited different Se-related changes in gene regulation and metabolism. Many genes involved in sulfur (S) uptake and assimilation were upregulated. Accordingly, Se treatment enhanced sulfate levels in plants, but the quantity of organic S metabolites decreased. Transcripts regulating the synthesis and signaling of ethylene and jasmonic acid were also upregulated by Se. Selenate appeared to repress plant development, as suggested by the down-regulation of genes involved in cell wall synthesis and auxin-regulated proteins. The Se-responsive genes discovered in this study may help create plants that can better tolerate and accumulate Se, which may enhance the effectiveness of Se phytoremediation or serve as Se-fortified food. Keywords: selenate, abiotic stress

2007-10-24 | GSE9311 | GEO

Opposite metabolic responses of shoots and roots to drought.

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| S-EPMC4212232 | biostudies-literature

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