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Transcriptional profiling of Arabidopsis shoots and roots responses to phytosulfokine (PSK)

ABSTRACT: Phytosulfokine (PSK) is a plant peptide hormone that contributes to plant signaling and induced various of effects, including growth, senescence, stress tolerant and defense responses. PSK is a pentapeptide with two tyrosine sulfated by tyrosylprotein sulfotransferase (TPST) enzyme. The sulfated PSK can bind to its cell surface receptors, but the transcriptional readouts remain largely unknown. Here, we treated tpst mutant that are unable to produce native sulfated PSK with synthetic active PSK to capture time-series and tissue-specific readouts of PSK signaling.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE254986 | GEO | 2024/10/17

REPOSITORIES: GEO

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Transcriptional profiling of Arabidopsis seedlings responses to phytosulfokine (PSK)

Project description:Phytosulfokine (PSK) is a plant peptide hormone that contributes to plant signaling and induced various of effects, including growth, senescence, stress tolerant and defense responses. PSK is a pentapeptide with two tyrosine sulfated by tyrosylprotein sulfotransferase (TPST) enzyme. The sulfated PSK can bind to its cell surface receptors, but the transcriptional readouts remain largely unknown. Here, we treated tpst mutant that are unable to produce native sulfated PSK with synthetic active PSK to capture concentration-sensitive readouts of PSK signaling.

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Next Generation Sequencing Facilitates Quantitative Analysis of PSKγ1ox and control Transcriptomes

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Project description:Phytosulfokine (PSK) is a plant pentapeptide hormone that fulfills a wide range of functions. Although it has been frequently reported to function in the inverse regulation of growth and defence in response to (hemi)biotrophic pathogens, the mechanism involved remains largely unknown. Using tomato (Solanum lycopersicum) and Pseudomonas syringae pv. tomato (Pst) DC3000 pathogen as a system, we present compelling evidence that the tomato PSK receptor PSKR1 interacts with the calcium-dependent protein kinase CPK28, which phosphorylates the key enzyme of nitrogen assimilation glutamine synthetase GS2 at two sites (S334 and S360 sites). These post translational modifications uncouple the two PSK-induced effects on defence responses and on growth regulation, respectively. The discovery of these sites will inform breeding strategies designed to optimize the growth-defence balance. The concept that such peptide hormones can regulate plant growth and resistance in a compatible manner is attractive, not least because crop yields and fitness can be optimized simultaneously in global climate-change era.

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Transcriptional profiling of Arabidopsis shoots and roots responses to phytosulfokine (PSK)

Project description:Transcriptional profiling of Arabidopsis shoots and roots responses to phytosulfokine (PSK)

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Transcriptional profiling of Arabidopsis responses to phytosulfokine (PSK)

Project description:Transcriptional profiling of Arabidopsis responses to phytosulfokine (PSK)

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Transcriptional profiling of Arabidopsis seedlings responses to phytosulfokine (PSK)

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