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Nasopharyngeal lymphatic plexus serves as a central hub for drainage of cerebrospinal fluid [Aged]

ABSTRACT: To investigate the heterogeneity of murine nasopharyngeal LECs, CD31+ PDPN+ LECs from the aged mouse nasopharynx were FACS sorted and single-cell RNA seq was performed

ORGANISM(S): Mus musculus

PROVIDER: GSE227324 | GEO | 2023/04/01

REPOSITORIES: GEO

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Nasopharyngeal lymphatic plexus serves as a central hub for drainage of cerebrospinal fluid [Adult]

Project description:To investigate the heterogeneity of murine nasopharyngeal LECs, CD31+ PDPN+ LECs from the adult mouse nasopharynx were FACS sorted and single-cell RNA seq was performed

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Project description:Objective was to uncover the transcriptional changes occuring in melanoma-associated LECs relative to LECs of naïve skin. To investigate the transcriptional changes in LECs during tumor development, RNA was extracted from LECs sorted from naïve skin and BPC (Tyr::Cre-ER, BRafV600E) murine melanomas and bulk RNA-seq performed. Naïve skin LECs were collected from 3 mice and tumor-associated LECs collected from 4 mice.

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Project description:In this analysis we have compared the gene expression profiles of lymphatic endothelial cells (LECs) isolated from human intestine (iLECs) versus LECs from human skin (dLECs).

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Project description:Human Notch1 intracellular domain (NICD) was overexpressed in human primary lymphatic endothelial cells (LECs) for 10 and 24 hours by adenovirus. A GFP-control adenovirus-infected cells (24hours) and uninfected cells were also analysed as controls. Total RNAs were harvested and subjected to Affymetrix U133A microarray. Human primary lymphatic endothelial cells (LECs) were isolated from human foreskin and cultured and expanded to population passages 5~6. Healthy subconfluent primary LECs were infected with adenovirus expressing human Notch1 intracellular domain (NICD) for 10 or 24 hours. In parallel, LECs were also infected with a GFP-expressing control adenovirus for 24 hours. Uninfected LECs were also used as a negative control in the same experiments

2010-03-20 | E-GEOD-20978 | biostudies-arrayexpress

Gene expression data of nasopharyngeal carcinoma (NPC) cell and Podoplanin (PDPN) siRNA treatment

Project description:Podoplanin, a transmembrane sialomucin-like glycoprotein, is now widely used as a marker for lymphatic endothelial cells and fibroblastic reticular cells in lymphoid organs, but its study in nasopharyngeal carcinoma (NPC) is still limited. The aims of this study were t characterize the role of PDPN in NPC. Our results showed that PDPN was expressed in most TW01 NPC cells. PDPN knockdown by siRNA decreased NPC cell proliferation, migration, and invasion. Knocking down PDPN results in suppression of NPC cell proliferation, migration, and invasion. PDPN may serve as a potential chemotherapeutic target for NPC treatment in the future.

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Podoplanin-expressing macrophages (PoEMs) promote lymphangiogenesis and favour lymphatic metastasis in breast cancer

Project description:RNAseq was performed on PoEMs (CD11b+, F4/80+, PDPN+) vs. non-PoEMs (CD11b+, F4/80+, PDPN-) sorted from orthotopically inoculated 4T1 tumors in WT mice.

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Single Cell Analysis of Lung Lymphatic Endothelial Cells and Lymphatic Responses during Influenza Infection

Project description:Tissue lymphatic vessels network plays critical roles in immune surveillance and tissue homeostasis in response to pathogen invasion, but how lymphatic system per se is remolded during infection is less understood. Here, we observed that influenza infection induces a significant increase of lymphatic vessel numbers in the lung, accompanied with extensive proliferation of lymphatic endothelial cells (LECs). Single-cell RNA sequencing illustrated the heterogeneity of LECs, identifying a novel PD-L1+ subpopulation that is present during viral infection but not at steady state. Specific deletion of Pd-l1 in LECs elevated the expansion of lymphatic vessel numbers during viral infection. Together these findings elucidate a dramatic expansion of lung lymphatic network in response to viral infection, and reveal a PD-L1+ LEC subpopulation that potentially modulates lymphatic vessel remolding.

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Single-cell transcriptomics of mouse lung lymphatic endothelial cells

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