Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative transcriptome analysis of testes and ovaries reveals sex-biased genes and pathways in zebrafish

ABSTRACT: The goals of this study are to compare the differentially expressed genes between testes and ovaries of zebrafish based on RNA-seq data and some of these genes were validated by qRT–PCR.Further, the differentially expressed genes were devided into up-regulated and down-regulated genes for GO and KEGG analysis.

ORGANISM(S): Danio rerio

PROVIDER: GSE227389 | GEO | 2024/01/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional signature of the developing rat testes and ovaries following embryonic exposure to 2,3,7,8-TCDD

Project description:We determined global gene changes in immature ovaries and testes in response to an in utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure. Microarray analysis was performed using testes and ovaries of the dioxin-exposed dams’ offspring. One hundred and thirteen genes were differentially expressed in ovaries and 56 genes in testes of 14 and 5 days-old, respectively. Real-time PCR was used to validate and extend data using RNA extracted from 5 to 145-day old rat testes and 3 to 25-day old rat ovaries. A single gene of the classic dioxin battery, i.e., the repressor of the aryl hydrocarbon receptor (Ahrr) was found altered in testes. In contrast, several of them including Cyp1a1, Cyp1b1, Nqo1, and Ahrr were found up-regulated in ovary, pituitary (a different endocrine organ) and liver. In addition to Ahrr, we identified 6 genes targeted by dioxin in both gonads, including the chemokines Cxcl4, Ccl5. Ccl5 gene expression levels were also regulated in pituitary and liver, so as pituitary Cxcl4. Four genes targeted by TCDD in testis and meeting stringent criteria were further surveyed. It included 2 genes with no previous reported function in testis, Art2b, Gzmf, Hpgds and Fgf13. Fgf13 was down-regulated in testis, and pituitary but not in ovary or liver. Interestingly, Art2b and Gzmf were up-regulated in testis, liver and pituitary but not ovary. Finally, Hpgds was unique in that expressed in various tissues it was regulated by TCDD in the gonads but not in the other tissues studied.

2011-10-12 | GSE32890 | GEO

Transcriptional signature of the developing rat testes and ovaries following embryonic exposure to 2,3,7,8-TCDD

2011-10-11 | E-GEOD-32890 | biostudies-arrayexpress

Global gene expression comparation among testes, ovaries, Smad4flox/floxMerCreMer Stra8+/− ovaries and Smad4flox/floxMerCreMer Stra8−/− ovaries

Project description:To confirm that female-to-male sexual fate reversal in Smad4flox/floxMerCreMer Stra8−/− ovaries occurs independently of somatic environment. We analyzed transcriptome of samples using RNA from control testes, ovaries, Smad4flox/floxMerCreMer Stra8+/− and Smad4flox/floxMerCreMer Stra8−/− ovaries.

2016-05-11 | GSE68773 | GEO

Comparative transcriptome analysis of testes and ovaries reveals sex-biased genes and pathways in zebrafish

Project description:Comparative transcriptome analysis of testes and ovaries reveals sex-biased genes and pathways in zebrafish

| PRJNA944944 | ENA

Transcription profiling of mouse ovaries lacking Foxl2, Wnt4 or Kit and wild type ovaries and testes

Project description:Comparison of Foxl2-null ovaries to wildtype ovaries, ovaries lacking Wnt4 or Kit, or testes, throughout mouse development. The goal of this study was to identify early Foxl2 target genes as well as other ovarian, anti-testis genes that may act independently of Foxl2. Experiment Overall Design: We studied 43 samples over 15 conditions to cover a wide range of wiltype and pathological states showing highly divergent alterations of cell type composition. This was meant to identify the most specific, cell context-independent targets of Foxl2.

2009-07-05 | E-GEOD-12905 | biostudies-arrayexpress

Identification and characterization of sex-biased microRNAs in Bactrocera dorsalis (Hendel)

Project description:We constructed four small RNA libraries from whole body of females, males (except ovaries and testes) and ovaries, testes of B. dorsalis for deep sequencing. The data analysis revealed 314 known and 221 novel miRNAs from these libraries. 14 female-biased and 12 male-biased miRNAs that may be involved in sexual differentiation were found by comparing the miRNA expression profiles in the four libraries.

2017-02-14 | GSE80536 | GEO

Transcription profiling of Drosophila testes, heads, ovaries and whole gonadectomized males to analyze transcription patterns in the testes

Project description:In order to analyze transcription pattern in Drosophila testes, we performed competitive hybridizations of testes-derived cDNA against the cDNAs obtained from heads, ovaries, and whole gonadectomized males.

2008-05-01 | E-TABM-273 | biostudies-arrayexpress

Transcriptome analysis of wildtype and sox3-/- zebrafish adult ovary

Project description:The goals of this study are to compare the differentially expressed genes between wildtype and sox3-/- zebrafish ovaries based on RNA-seq data and some of these genes were validated by qRT–PCR. Further, the differentially expressed genes were devided into up-regulated and down-regulated genes for GO and KEGG analysis.

2019-04-25 | GSE115806 | GEO

Expression data from Foxl2 wild-type and mutant ovaries and testes

Project description:Foxl2 is a forkhead transcription factor expressed only in the female, but not in the male gonad. We have created mice homozygous mutant for the Foxl2 gene (KO) as well as mice carrying a conditional mutant Foxl2 allele (floxed). The expression profiles of conventional Foxl2 knockout and wildtype ovaries were compared at P3, using the Affy Mouse Genome 430 2.0 Array. Adult wildtype and conditional mutant (Foxl2 floxed x RosaCre-EBD treated with tamoxifen) ovaries were compared to adult wildtype testes using the Affymetrix Mouse Gene 1.0 ST Array. Both experiments (KO/WT P3 and Mutant/WT/Testis Adult were also compared to each other.)

2010-01-01 | GSE16853 | GEO

The genomic distribution of sex-biased genes in Drosophila serrata: X-chromosome demasculanisation, feminisation, and hyper-expression in both sexes

Project description:Data from whole-body, head, thorax, abdomen, ovaries, testes and accessory glands was used to assess possible causes of a non-random distribution of sex-biased genes (sexually dimorphic expression). All samples were derived from virgin adult flies.

2013-11-05 | GSE45801 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data