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A Xenopus DNA microarray approach to identify novel direct BMP target genes involved in early embryonic development

ABSTRACT: Bone Morphogenetic Proteins (BMPs), a subgroup of the TGF-beta superfamily, were originally isolated from bone on the basis of their ability to induce ectopic bone development. While BMPs are involved in a wide range of developmental and physiological functions, very few vertebrate target genes in this pathway have been identified. To identify target genes regulated by the BMP growth factor family in Xenopus, large-scale microarray analyses were conducted to discover genes directly activated by this factor in dissociated animal cap tissues treated with a combination of the protein synthesis inhibitor cycloheximide and BMP2. Keywords = Xenopus Keywords = BMP Keywords = microarray Keywords = development Keywords = target genes Keywords: parallel sample

ORGANISM(S): Xenopus laevis

PROVIDER: GSE2274 | GEO | 2005/03/05

SECONDARY ACCESSION(S): PRJNA91457

REPOSITORIES: GEO

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A Xenopus DNA microarray approach to identify novel direct BMP target genes involved in early embryonic development

Project description:Bone Morphogenetic Proteins (BMPs), a subgroup of the TGF-ï¢ superfamily, were originally isolated from bone on the basis of their ability to induce ectopic bone development. While BMPs are involved in a wide range of developmental and physiological functions, very few vertebrate target genes in this pathway have been identified. To identify target genes regulated by the BMP growth factor family in Xenopus, large-scale microarray analyses were conducted to discover genes directly activated by this factor in dissociated animal cap tissues treated with a combination of the protein synthesis inhibitor cycloheximide and BMP2. Keywords = Xenopus Keywords = BMP Keywords = microarray Keywords = development Keywords = target genes

2010-06-10 | E-GEOD-2274 | biostudies-arrayexpress

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Project description:Bone morphogenetic proteins (BMPs) regulate many aspects of skeletal development, including osteoblast and chondrocyte differentiation, cartilage and bone formation, and cranial and limb development. Among them, BMP2, one of the most potent osteogenic signaling molecules, stimulates osteoblast differentiation, while it inhibits myogenic differentiation in C2C12 cells. We used cDNA microarrays to elucidate regulators of BMP-2-induced osteoblast differentiation.

2011-11-11 | E-GEOD-33567 | biostudies-arrayexpress

Microarray on osteoblasts made from bone marrow stromal cells from Bmp2 flox/flox and Bmp2; Prx1-Cre mice

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