Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of depletion of USF1 or USF2 on Jurkat Tat mHIV-Luciferase T cell gene expression.

ABSTRACT: To investigate the cooperative function USF factors for the regulation of T cell transcriptom, we established Jurkat mHIV-Luciferase cells in which USF1 or USF2 was knocked out by CRISPR-Cas9. We then performed gene expression profiling analysis using data obtained from RNA-seq of cell lines either left untreated or stimulated by PMA and Ionomycin co-treatment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE227850 | GEO | 2023/06/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Effect of depletion of USF1 or USF2 on Jurkat Tat mHIV-Luciferase T cell gene expression.

Project description:Effect of depletion of USF1 or USF2 on Jurkat Tat mHIV-Luciferase T cell gene expression.

| PRJNA947182 | ENA

Chromatin immunoprecipitation of human cell line HepG2 with anti-Histone H3 acetyl K9/14, USF1 antibody, USF2 antibody and normal rabbit IgG

Project description:Whole genome ChIP-chip study using human liver cell line HepG2 and anti-Histone H3 acetyl K9/14 (06-599); USF1 antibody (H-86, sc-8983); USF2 antibody (C-20, sc-862) and normal rabbit IgG (12-370). Three completely independent biological replicates were performed for each antibody, obtaining the corresponding input as total genomic DNA reference. Hybridizations were performed using Affymetrix GeneChip Human Tiling 2.0R Array set (7 arrays set).

2007-11-30 | E-TABM-314 | biostudies-arrayexpress

USF2 interacted proteins in HEK293T cells

Project description:We found that USF2 is repressor of lysosomal genes. To find the interacting co-repressor complex of USF2, we performed USF2 complex purification and mass spectrography.

2024-09-11 | PXD050434 | Pride

Effects of KD USF2 on gene expressions in C2C12

Project description:USF2 has emerged as a key player in various cellular processes, including cell cycle regulation， apoptosi, metabolism, and response to cellular stress. In the context of muscle biology, the role of USF2 is not clear. To elucidate the functional significance of USF2, we conducted knockdown (KD) experiments targeting USF2 in C2C12 myoblast cells. The KD of USF2 resulted in the differential expression of a set of genes, and functional enrichment analysis of genes affected by KD of USF2 were performed.

2024-01-01 | GSE244161 | GEO

Overexpression of USF1 in HEK293T cells

2009-09-14 | GSE17300 | GEO

Overexpression of USF1 in HEK293T cells

Project description:Overexpression of USF1 in HEK293T cells in vitro to ascertain the genes downstream of USF1. Will identify direct targets as well as indirect targets of USF1. Keywords: Overexpression of transcription factor to determine downstream targets. Cloned USF1 from cDNA and transiently transfected into HEK293T cells. Total RNA collected 48 hours after transfection. Empty vector used as control. Expression of USF1 was validated using western blot, and qRT-PCR was used to validate differential expression.

2009-09-14 | E-GEOD-17300 | biostudies-arrayexpress

USF1 haplotype comparison

Project description:Comparison of gene expression for individuals affected with FCHL exhibiting the USF1 susceptibility haplotype and FCHL affected indiviuals carrying the protective haplotype Keywords: ordered

2004-02-29 | GSE590 | GEO

Genome binding/occupancy profiling of USF2 under glucose starvation and changes in H3K27ac based on the presence of USF2

Project description:Intricate regulation of lysosome and autophagy processes is essential for maintaining cellular homeostasis and basal metabolism. While the consequences of disrupting or attenuating lysosome and autophagy systems have been extensively studied, little is known about the impact of hyper-activation of lysosomal and autophagy genes on homeostasis. Our research uncovers previously unknown transcriptional repression mechanism by upstream stimulatory factor 2 (USF2), which inhibits lysosomal and autophagy genes in nutrient-rich conditions. USF2 binds to the CLEAR motif within lysosomal genes along with HDAC1, which diminishes H3K27 acetylation levels, restrains chromatin accessibility, and lowers lysosomal gene expression. Under starvation, USF2 competes with TFEB, a master transcriptional activator of lysosomal and autophagy genes, to bind target gene promoters in phosphorylation-dependent manner. Phosphorylation of the S155 site by GSK3b plays a pivotal role in controlling USF2's DNA binding activity for the repression of lysosomal genes while GSK3b-mediated phosphorylation of TFEB enhances its cytoplasmic retention. Applying these discoveries has potential for treating diseases associated with protein aggregation, including alpha-1 antitrypsin deficiency. These findings demonstrate that the USF2 repression mechanism could be a potent therapeutic strategy for various lysosome and autophagy-related diseases.

2024-08-06 | GSE252310 | GEO

Effect of PMA/Ionomycin mediated T cell activation on Jurkat Tat gene expression

Project description:To investigate gene regulation in response to T cell activation. We then performed gene expression profiling analysis using data obtained from RNA-seq of cell lines stimulated by PMA and Ionomycin.

2022-12-28 | GSE221851 | GEO

Genome-wide analysis of USF1-associated differential gene expression in mouse liver

Project description:Analysis of adenovirus-mediated USF1 overexpression in liver at gene expression level. Results provide important information of the response of mouse liver to USF1 overexpression, such as specific USF1-induced, suppressed, up- or down-regulated genes invovled in metabolism and immune responses.

2010-03-31 | GSE17442 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data