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ChIP-seq of holocentric Chionographis japonica to determine the centromere distribution and genome organization

ABSTRACT: To determine the distribution of centromere units in the genome of holocentric Chionographis japonica, we performed CENH3-ChIPseq using the customized species-specific CENH3 antibody. We mixed the chromatins of C. japonica and Secale cereal (inbred line Lo7) to dilute the highly abundant centromeric Chio satellite repeats (16%) in the C. japonica genome before immunoprecipitation. In addition, to determine the large-scale genome organization, we performed ChIPseq by targeting the evolutionarily conserved eu- and heterochromatin-specific histone marks H3K4me2 and H33K9me2

ORGANISM(S): Secale cereale Chionographis japonica

PROVIDER: GSE228407 | GEO | 2023/05/08

REPOSITORIES: GEO

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