Dataset Information

The skeletal proteome of the feather star Anneissia japonica

ABSTRACT: Skeletal elements from the feather star Anneissia japonica were isolated and stripped of organic material. The skeleton was then demineralized and the occluded proteins isolated. The proteins were separated by SDS-PAGE and fractions were analyzed by LC-MS/MS. The results were compared to predicted proteins encoded by the genome. The proteins found in the organic matrix of the skeleton were compared to those found in other skeletons both within Echinodermata and to other taxa.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Anneissia Japonica

SUBMITTER: Michael Ford

LAB HEAD: Brian Livingston

PROVIDER: PXD019526 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Genomic insights of body plan transitions from bilateral to pentameral symmetry in Echinoderms.

Li Yongxin Y Omori Akihito A Flores Rachel L RL Satterfield Sheri S Nguyen Christine C Ota Tatsuya T Tsurugaya Toko T Ikuta Tetsuro T Ikeo Kazuho K Kikuchi Mani M Leong Jason C K JCK Reich Adrian A Hao Meng M Wan Wenting W Dong Yang Y Ren Yaondong Y Zhang Si S Zeng Tao T Uesaka Masahiro M Uchida Yui Y Li Xueyan X Shibata Tomoko F TF Bino Takahiro T Ogawa Kota K Shigenobu Shuji S Kondo Mariko M Wang Fayou F Chen Luonan L Wessel Gary G Saiga Hidetoshi H Cameron R Andrew RA Livingston Brian B Bradham Cynthia C Wang Wen W Irie Naoki N

Communications biology 20200710 1

Echinoderms are an exceptional group of bilaterians that develop pentameral adult symmetry from a bilaterally symmetric larva. However, the genetic basis in evolution and development of this unique transformation remains to be clarified. Here we report newly sequenced genomes, developmental transcriptomes, and proteomes of diverse echinoderms including the green sea urchin (L. variegatus), a sea cucumber (A. japonicus), and with particular emphasis on a sister group of the earliest-diverged echi ...[more]

PMID: 32651448

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Project description:The process of calcium carbonate biomineralization has arisen multiple times during metazoan evolution. In the phylum Cnidaria, biomineralization has mostly been studied in the subclass Hexacorallia (i.e. stony corals) in comparison to the subclass Octocorallia (i.e. red corals); the two diverged approximately 600 million years ago. The precious Mediterranean red coral, Corallium rubrum, is an octocorallian species, which produces two distinct high-magnesium calcite biominerals, the axial skeleton and the sclerites. In order to gain insight into the red coral biomineralization process and cnidarian biomineralization evolution, we studied the protein repertoire forming the organic matrix (OM) of its two biominerals. We combined High-Resolution Mass Spectrometry and transcriptome analysis to study the OM composition of the axial skeleton and the sclerites. We identified a total of 102 OM proteins, 52 are shared between the two red coral biominerals with scleritin being the most abundant protein in each fraction. Contrary to reef building corals, the red coral is collagen-rich (10 collagen-like proteins). Agrin-like glycoproteins and proteins with sugar-binding domains are also predominant. Twenty-seven and 23 proteins were uniquely assigned to the axial skeleton and the sclerites, respectively. Their inferred regulatory function suggests that the difference between the two biominerals rather relies on the modeling of the matrix network than on specific structural components. At least one OM component appears to have been horizontally transferred from prokaryotes early during Octocorallia evolution. Our results support the view that calcification of the red coral axial skeleton likely represents a secondary calcification of an ancestral gorgonian horny axis. In addition, the comparison with stony coral skeletomes highlighted the low proportion of similar proteins between the biomineral OMs of hexacorallian and octocorallian corals, suggesting an independent acquisition of calcification in anthozoans.

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