Project description:The addition of 2ME2 (HIF-1 inhibitor) to rat model of galactose-induced cataract decreased opacity and therapeutic effect. Genes regulated by 2ME2 addition were identified by microarray analysis. A total of 7 samples were analyzed: samples with no cataract (Control_Day4, Control_Day6), samples with cataract (Galactose-1, Galactose-2, Galactose-3), samples with decreased lens opacity (2ME2, 2ME2-2)
Project description:Lens development exhibits a seamless progression from embryogenesis through ageing. Lens maturation and growth proceed throughout life; lens fibre cells differentiate from lens epithelial cells at the equator of the lens, migrate inwards at the bow region and mature around pre-existing fibre cells at the centre of the lens. Lens fibre cells lose their nuclei via an apoptosis like mechanism as they mature. We therefore decided to study further the relationship of apoptosis related gene expression during vital stages of lens maturation in the mouse. This is an important question as defects in lens function and/or maturation result in clouding of the lens, or cataract, which can result in considerable visual impairment. Keywords: development/age comparison
Project description:We created a rat sugar cataract model and examined the effects of various inhibitors on lens clouding. Lenses were removed from 6-week-old SD rats and cultured in M199 medium containing 30 mM galactose.
