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Microarray data of lenses with 2ME2

ABSTRACT: The addition of 2ME2 (HIF-1 inhibitor) to rat model of galactose-induced cataract decreased opacity and therapeutic effect. Genes regulated by 2ME2 addition were identified by microarray analysis. A total of 7 samples were analyzed: samples with no cataract (Control_Day4, Control_Day6), samples with cataract (Galactose-1, Galactose-2, Galactose-3), samples with decreased lens opacity (2ME2, 2ME2-2)

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE240617 | GEO | 2024/02/16

REPOSITORIES: GEO

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Expression data from Sprague Dawley rat lenses cultured for 4 days - addition of various inhibitors

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Microarray data of lenses with glutamate

Project description:The addition of Glutamate to the lens induced by galactose eliminated the white clouding formed and showed a therapeutic effect. Genes regulated by Glutamate addition were identified by microarray analysis. A total of seven samples were used in this analysis: two samples without galactose (control), three samples with galactose (galactose) and two samples with Glutamate (Glutamate).

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Expression data from lens epithelial cells (LECs) from Shumiya cataract rats (SCR) with or without cataract (Cat+ or Cat-)

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Project description:By adding MMS or Bleomycin, a DNA damage inducing agent, to the removed rat lens and culturing it, opacity was formed in the lens. To investigate genes whose expression fluctuates when DNA damage is inflicted and causes lens opacity, microarray analysis was performed.

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Pyruvate is effective in restoring cataracts formed by galactose-induced cataract models.

Project description:The addition of Pyruvate to the lens induced by galactose eliminated the white clouding formed and showed a therapeutic effect. Genes regulated by pyruvate addition were identified by microarray analysis. A total of seven samples were used in this analysis: two samples without galactose (control), three samples with galactose (galactose) and two samples with Pyruvate (Pyruvate).

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Transcription profiling of mouse to identify strain-specific variation and the onset of gap junction mutation-induced cataractsc

Project description:Disruption of the mouse gene encoding the gap junction subunit alpha3 connexin 46 (Cx46) results in the formation of lens cataracts. The timing of the onset of this lens opacity is affected by the genetic background, i.e. the mouse strain. To elucidate the mechanism by which cataracts form in the 129Sv/Jae strain earlier than in the C57BL/6J strain, global gene expression was quantitated in the lenses of these strains. Lens cDNAs were analyzed by hybridization to DNA microarrays and with real time-PCR. Theories are proposed based on the observed higher level of expression of the stress-response genes in the C57BL/6J strain and variations in the expression levels of genes involved in protein synthesis, metabolism, catabolism and cell proliferation. How these variations in gene expression might affect the response of lens fiber cells to the increased calcium, caused by lack of alpha3Cx46, is considered. The possibility that the proteins coded by the strain-variable genes might influence the cataract-associated proteolysis of gamma-crystallin is also addressed. Experiment Overall Design: To determine differences in transcript expression between the lenses of two mouse wild type strains (129 SvJae and C57BL/6J), as well as between alpha3Cx46 KO and wild-type mice. The former comparison may lead to identification of potential candidate(s) genes that prevent (or promote) cataract formation, whereas the latter comparison may provide insights into the mechanism by which cataract formation occurs in the alpha3Cx46 KO mice. Total 8 samples were used ( two separate samples for each of the following 4 types of mice: 129SvJae wild type, 129SvJae alpha3Cx46 KO, C57BL/6J wild type and C57BL/6J alpha3Cx46 KO)

2009-05-11 | E-GEOD-5645 | biostudies-arrayexpress

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