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Single cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional and cellular changes in response to Borrelia burgdorferi infection

ABSTRACT: Borreliella burgdorferi is the causative agent of Lyme disease, which is the most common vector-borne disease in the United States. While cases of Lyme disease are geographically limited to the Northeast, mid-Atlantic, and Midwest states, there are still estimates of almost 500,000 cases annually in the United States alone. In this study, we sought to assess transcriptional changes that occur in response to B. burgdorferi infection in the ankle joints of C57BL/6 mice. To this end, we performed single cell RNA sequencing (scRNA-seq) to transcriptionally profile cells, identify population heterogeneity, and analyze how cellular environments change over time. Sequencing of immune and non-immune cells in the mouse ankle joint was performed over five distinct time points, starting with uninfected animals and progressing to eight weeks post-infection in two-week increments.

ORGANISM(S): Mus musculus

PROVIDER: GSE233850 | GEO | 2023/11/01

REPOSITORIES: GEO

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