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Expression profiling of METTL14 depleted human pancreatic β-like cells

ABSTRACT: N6-methyladenosine (m6A) is the most abundant chemical modification in mRNA, and plays important roles in human embryonic stem cell pluripotency, maintenance, and differentiation. However, the role of m6A and the precise mechanisms involved during the development of β-cells are unexplored. Here, we explored the requirement of METTL14-mediated m6A RNA methylome in human pancreatic β-like cells by simultaneously inducing METTL14 knockdown in H1 human embryonic stem cells (hESCs) with doxycycline (Dox) treatment and differentiating hESCs towards pancreatic β-like cells in vitro.

ORGANISM(S): Homo sapiens

PROVIDER: GSE236323 | GEO | 2024/06/13

REPOSITORIES: GEO

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Expression profiling of METTL14 depleted human embryoid bodies

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