Sigle-cell transcriptome analysis of EpiSC-derived neural stem cell lines derived from EpiSCs under different Wnt signal inputs

ABSTRACT: The four neural stem cell lines, XN4, XN1, KN1, and CN4, were derived from EpiSCs under different Wnt signal inputs (Nakamura et al., to be submitted). Microarray analysis of the transcripts indicated that these cells have different anteroposterior regional characteristics of the embryonic CNS from the forebrain to the thoracic spinal cord. However, each cell line has a relatively broad anteroposterior regional coverage. For instance, the XN1 line expressed midbrain-characteristic genes and hindbrain-characteristic genes. Single-cell transcriptome analysis of the four cell lines was performed to confirm that the broad anteroposterior specificity is intrinsic to individual cells of the cell line rather than the cell lines are composite of separable subpopulations with narrower anteroposterior specificities.

ORGANISM(S): Mus musculus

PROVIDER: GSE239383 | GEO | 2023/08/01

REPOSITORIES: GEO