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SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [HEY1 ChIP-seq]

ABSTRACT: Our previous results from RNA-seq and IP-MS together suggest that SUMOylation may be critical for the DNA-binding capability of HEY1 to enhance its transcriptional repressive activity. To confirm our speculation, we conducted ChIP assays in HUVECs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE241148 | GEO | 2024/01/10

REPOSITORIES: GEO

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SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [MLEC RNA-seq]

Project description:To validate the results in primary endothelial cells including HUVECs and HMVECs, the effect of HEY1 SUMOylation on angiogenic functions was examined in MLECs isolated form Hey1 iEC-KO HEY1-WT/3KR iEC-KI mice.

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SUMOylation fine tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis

Project description:Reversible HEY1 SUMOylation is a molecular mechanism that coordinates endothelial angiogenic signaling and angiogenesis, both in physiological and pathological milieus, by fine tuning the transcriptional activity of HEY1. Specifically, S UMOylation facilitates the formation of the HEY1 transcriptional complex and enhances its DNA binding capability in endothelial cells.

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SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [HUVEC RNA-seq]

Project description:Previous studies have shown that HEY1 is a major downstream transducer of NOTCH signaling and transcriptionally represses VEGFR2. We aimed to investigate whether SUMOylation is critical for HEY1-mediated transcriptional regulation of angiogenesis in endothelial cells. To address this question, we performed RNA-seq to assess the repressive landscape of HEY1-WT and HEY1-3KR.

2024-01-10 | GSE241150 | GEO

SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [HEY1 ChIP-seq]

Project description:SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [HEY1 ChIP-seq]

| PRJNA1006648 | ENA

SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis

Project description:SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis

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SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [HUVEC RNA-seq]

Project description:SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [HUVEC RNA-seq]

| PRJNA1006647 | ENA

SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [MLEC RNA-seq]

Project description:SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [MLEC RNA-seq]

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SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-01-10 | GSE241152 | GEO

Yeast ORC sumoylation status fine-tunes origin activity

Project description:Sumoylation is emerging as a post-translation modification important for chromosome duplication and stability. The origin recognition complex (ORC), which directs DNA replication initiation by loading the MCM replicative helicases onto origins, is sumoylated in both yeast and human cells. However, the biological consequences of ORC sumoylation are largely unclear. Here we report the effects of hyper- and hypo-sumoylation of yeast ORC using multiple approaches. We show that ORC hyper-sumoylation preferentially reduces the activity of a subset of early origins, while Orc2 hypo-sumoylation has an opposing effect. Mechanistically, ORC hyper-sumoylation leads to reduced MCM loading in vitro and diminished MCM chromatin association in vivo. The importance of an appropriate level of ORC sumoylation is suggested by the data that either hyper- or hypo-sumoylation of ORC results in genome instability and a dependence on other genome maintenance factors for cell fitness. Thus, yeast ORC sumoylation status needs to be fine-tuned to achieve optimal origin activity control and genome stability.

2023-03-11 | PXD032977 | Pride

Yeast ORC sumoylation status fine-tunes origin licensing.

Project description:Sumoylation is emerging as a posttranslation modification important for regulating chromosome duplication and stability. The origin recognition complex (ORC) that directs DNA replication initiation by loading the MCM replicative helicase onto origins is sumoylated in both yeast and human cells. However, the biological consequences of ORC sumoylation are unclear. Here we report the effects of hypersumoylation and hyposumoylation of yeast ORC on ORC activity and origin function using multiple approaches. ORC hypersumoylation preferentially reduced the function of a subset of early origins, while Orc2 hyposumoylation had an opposing effect. Mechanistically, ORC hypersumoylation reduced MCM loading in vitro and diminished MCM chromatin association in vivo. Either hypersumoylation or hyposumoylation of ORC resulted in genome instability and the dependence of yeast on other genome maintenance factors, providing evidence that appropriate ORC sumoylation levels are important for cell fitness. Thus, yeast ORC sumoylation status must be properly controlled to achieve optimal origin function across the genome and genome stability.

| S-EPMC9480853 | biostudies-literature

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