Genomics

Dataset Information

0

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [ChIPseq_786O]


ABSTRACT: HNF1B (Hepatocyte Nuclear Factor 1-Beta) is a POU-homeobox transcription factor expressed in kidney and frequently mutated in patients suffering from Congenital Anomalies of the Kidney and the Urinary Tract (CAKUT). HNF1B is a bookmarking factor, able to bind mitotic chromatin and necessary for the post-mitotic reactivation of gene expression. In order to characterize the molecular functions of HNF1B as a bookmarking factor, we performed ChIPseq on asynchronous and mitotic renal cells. We developed a novel ChIP method with a non-aqueous crosslinking (NAQC) to circumvent the well-known artefactual effects of formaldehyde crosslinking of mitotic chromosomes. NAQC-ChIP allowed the retention of the mitotic signal that was lost with a regular ChIP. With this novel methodology, we show that the vast majority HNF1B binding sites detected in asynchronous cells are also bound in mitosis.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE245086 | GEO | 2024/10/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-10-01 | GSE245070 | GEO
2024-10-01 | GSE245072 | GEO
2024-10-01 | GSE245071 | GEO
2024-04-29 | E-MTAB-12822 | biostudies-arrayexpress
2024-04-29 | E-MTAB-12824 | biostudies-arrayexpress
2020-07-14 | GSE121370 | GEO
2022-12-06 | GSE220253 | GEO
2023-12-31 | GSE240072 | GEO
2023-11-20 | PXD038950 | Pride
2023-05-01 | GSE223312 | GEO