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Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B

ABSTRACT: Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B

PROVIDER: PRJNA1026917 | ENA |

REPOSITORIES: ENA

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Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [ChIPseq_786O]

Project description:Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [ChIPseq_786O]

| PRJNA1026922 | ENA

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [RNAseq_786O]

Project description:Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [RNAseq_786O]

| PRJNA1026902 | ENA

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [ChIPseq_KEC]

Project description:Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [ChIPseq_KEC]

| PRJNA1026898 | ENA

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [RNAseq_KEC]

Project description:Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [RNAseq_KEC]

| PRJNA1026901 | ENA

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [RNAseq_KEC]

Project description:HNF1B (Hepatocyte Nuclear Factor 1-Beta) is a POU-homeobox transcription factor expressed in kidney and frequently mutated in patients suffering from Congenital Anomalies of the Kidney and the Urinary Tract (CAKUT). HNF1B is a bookmarking factor, able to bind mitotic chromatin and necessary for the post-mitotic reactivation of gene expression. In order to characterize the molecular functions of HNF1B as a bookmarking factor, we performed ChIPseq on asynchronous and mitotic renal cells. We developed a novel ChIP method with a non-aqueous crosslinking (NAQC) to circumvent the well-known artefactual effects of formaldehyde crosslinking of mitotic chromosomes. NAQC-ChIP allowed the retention of the mitotic signal that was lost with a regular ChIP. With this novel methodology, we show that the vast majority HNF1B binding sites detected in asynchronous cells are also bound in mitosis.

2024-10-01 | GSE245072 | GEO

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [RNAseq_786O]

2024-10-01 | GSE245071 | GEO

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [ChIPseq_KEC]

2024-10-01 | GSE245070 | GEO

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B [ChIPseq_786O]

2024-10-01 | GSE245086 | GEO

Topoisomerase I (TOP1) is a functional mitotic interactor of the bookmarking factor HNF1B

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-10-01 | GSE245087 | GEO

RNA Interacts with Topoisomerase I to Modulate its Catalytic Activity

Project description:Topoisomerase I (TOP1) is an essential enzyme that relaxes DNA to prevent and dissipate torsional stress during transcription. However, the mechanisms underlying the regulation of TOP1 activity remain elusive. Using eCLIP and UV-RIP-seq we identify TOP1 as a RNA binding protein (RBP). Using a TOP1 RNA binding mutant and TOP1cc-seq to map TOP1 catalytic activity, we reveal that RNA opposes TOP1 activity as RNAPII commences transcription of active genes. These findings support new insights into the coordinated actions of RNA and TOP1 in regulating DNA topological stress intrinsic to RNAPII-dependent transcription.

2024-08-21 | GSE213403 | GEO

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