Evaluation of the effect of maternal immune activation and juvenile distress on hypothalamus of 60 day old pigs
Ontology highlight
ABSTRACT: Female and male pigs were studied. Half of the pigs were exposed to immune activation from virally-infected mothers, and half were not exposed. At 60 days of age about one third of the pigs were given metabolic distress (fasting), one third of the pigs were given immune distress (poly(I:C)), and one third of the pigs were given Saline (control).
Project description:Female and male pigs were studied. Half of the pigs were exposed to immune activation from virally-infected mothers, and half were not exposed. At 21 days of age half the pigs were weaned and the remainder remained with the sow.
Project description:Female and male 21 days of age pigs were studied. Half of the pigs were prenatally exposed to immune activation from virally-infected gilts, and half were not exposed.
Project description:Female and male pigs were studied. Half of the pigs were exposed to immune activation from virally-infected mothers, and half were not exposed. At 17 days of age half the pigs were injected with morphine for 4 days and the remainder with saline.
Project description:Respiratory distress is one of the major causes of the high mortality rate in neonatal cloned animals. Although some therapeutic methods have been used to improve the survival of cloned neonatal animals, the mechanisms of their neonatal respiratory distress lacked thorough investigation. Pathological analyses including necropsy and histology were implemented to determine the precise disease phenotypes, by which not fully dilated lungs, alveolar collapse and thickened alveolar walls were detected in neonatal cloned cows dying of respiratory distress compared to naturally conceived neonatal cows. In addition, we compared mRNA expression profiles between the two groups and differentially expressed genes (DEGs) have been achieved. Based on DEGs, GO and KEGG pathway enrichment analyses were performed, which showed that processes and pathways associated with surfactant homeostasis were significantly enriched between the two groups (p < 0.05).
Project description:Acute Respiratory Distress Syndrome (ARDS) continues to have a high mortality. The objective of this study is to understand the differences in disease biology between survivors and non-survivors by characterizing BALF protein expression profiles in individual ARDS subjects.
Project description:Weaning is a stressor that can have significant impacts on health and physiology of the offspring. The effect of this stressor may be modulated by the exposure to other stressors during gestation such as the inflammatory signals of the mother in response to infection. The goal of this study is to determine the combined effects of weaning stress and immune stress during gestation on gene expression in the amygdala, a brain region critical for decision-making and aggression. A RNA-seq study of the amygdala of female and male pigs was undertaken. Within sex group, half of the pigs were weaned at 21 d of age and the rest remain with the gilts and were nursed. Also, half of the pigs in each group were raised from gilts exposed to a viral infection and half were raised by gilts that served as control.
Project description:Right ventricular failure was induced thourgh pulmonary banding in 11 pigs. Right ventricular failure was defined as a SRVP >50 mmHg during two hours. After right ventricular failure was induced, half the pigs were treated with a Glenn-shunt combined with pulmonary banding for one hour, and the other half served as control group with pulmonary banding only. The aim was to study the change in global gene expression during right ventricular failure due to pulmonary banding, and the effect of volume unloading during pulmonary banding.
Project description:Right ventricular failure was induced thourgh pulmonary banding in 11 pigs. Right ventricular failure was defined as a SRVP >50 mmHg during two hours. After right ventricular failure was induced, half the pigs were treatmed with a Glenn-shunt combined with pulmonary banding for one hour, and the other half served as control group with pulmonary banding only. The aim was to study the change in global gene expression during right ventricular failure due to pulmonary banding, and the effect of volume unloading during pulmonary banding. 11 pigs. Samples at the following time periods: 1) Baseline 2) Right ventricular failure 3) Treatment with modified Glenn-shunt/Control. After Right ventricular failure, pigs were divided into two groups a) Treatment with modified Glenn-shunt or b) Control group
Project description:Mesenchymal stromal cells are a potential therapeutic for Acute Respiratory Distress Syndrome due to COVID-19, with pleiotropic immunomodulatory and reparative properties.This study investigated the safety and efficacy of ORBCEL-C (CD362 enriched umbilical cord-derived Mesenchymal Stromal Cells) in this patient population.