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Coordinated Regulation of Myonuclear DNA Methylation, mRNA, and miRNA Levels Associates with the Metabolic Response to Rapid Synergist Ablation-Induced Skeletal Muscle Hypertrophy in Female Mice

ABSTRACT: We investigatedmyonucleus-specific microRNA transcriptomes following an acute hypertrophic stimulus in mouse plantaris.

ORGANISM(S): Mus musculus

PROVIDER: GSE245715 | GEO | 2023/11/01

REPOSITORIES: GEO

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Coordinated Regulation of Myonuclear DNA Methylation, mRNA, and miRNA Levels Associates With the Metabolic Response to Rapid Synergist Ablation-Induced Skeletal Muscle Hypertrophy in Female Mice.

Project description:The central dogma of molecular biology dictates the general flow of molecular information from DNA that leads to a functional cellular outcome. In skeletal muscle fibers, the extent to which global myonuclear transcriptional alterations, accounting for epigenetic and post-transcriptional influences, contribute to an adaptive stress response is not clearly defined. In this investigation, we leveraged an integrated analysis of the myonucleus-specific DNA methylome and transcriptome, as well as myonuclear small RNA profiling to molecularly define the early phase of skeletal muscle fiber hypertrophy. The analysis of myonucleus-specific mature microRNA and other small RNA species provides new directions for exploring muscle adaptation and complemented the methylation and transcriptional information. Our integrated multi-omics interrogation revealed a coordinated myonuclear molecular landscape during muscle loading that coincides with an acute and rapid reduction of oxidative metabolism. This response may favor a biosynthesis-oriented metabolic program that supports rapid hypertrophic growth.

| S-EPMC10666992 | biostudies-literature

Expression data during plantaris muscle hypertrophy induced by synergist ablation in young adult mice

Project description:Global gene expression patterns were determined from microarray results on day 1, 3, 5, 7, 10 and 14 during plantaris muscle hypertrophy induced by synergist ablation in young adult mice (5 months). For each time point, two Affymetrix chips (Mouse Gene 1.0 ST) were used with 100 ng of total RNA derived from a pooled sample of either the right or left plantaris muscles from six animals.

2013-05-21 | E-GEOD-47098 | biostudies-arrayexpress

Expression data during plantaris muscle hypertrophy induced by synergist ablation in young adult mice

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Expression data from muscle samples following synergist ablation

Project description:We used microarrays to detail the program of gene expression underlying the growth of the plantaris muscle following synergist ablation-induced supraphysiological overload

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Expression data during plantaris muscle hypertrophy with/without satellite cells induced by synergist ablation in young adult Pax7-DTA mice

Project description:Global gene expression patterns were determined from microarray results from sham surgery or following 1 week of plantaris muscle hypertrophy induced by synergist ablation in young adult Pax7-DTA mice (4 months). Vehicle treated mice have their full complement of satellite cells; tamoxifen treated mice have had their satellite cells genetically depleted through Cre-loxP technology After sham surgery or 1 week of overload, Affymetrix chips (mouse430_2.0) were used with 1 Âµg of total RNA derived from a pooled sample of the right and left plantaris muscles from 11 animals.

2014-02-20 | E-GEOD-52597 | biostudies-arrayexpress

Expression data during plantaris muscle hypertrophy with/without satellite cells induced by synergist ablation in young adult Pax7-DTA mice

2014-02-20 | GSE52597 | GEO

Transcriptome analysis following an acute loading-induced skeletal muscle growth stimulus in mice yields tools for discerning global and MYC regulatory networks

Project description:We investigated global, actively transcribed, stable, and myonucleus-specific transcriptomes following an acute hypertrophic stimulus in mouse plantaris. With these datasets we define global and Myc-specific dynamics at the onset of rapid loading-induced muscle fiber growth.

2023-05-01 | GSE213406 | GEO

The effect of age on the skeletal muscle transcriptome during hypertrophy

Project description:Transcriptome analysis of skeletal muscle during hypertrophic growth in aged mice Global gene expression patterns were determined from microarray results on day 1, 3, 5, 7, 10 and 14 during plantaris muscle hypertrophy induced by synergist ablation in old adult mice (25 months).

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Senolytics rescue blunted muscle hypertrophy in old mice

Project description:With aging, skeletal muscle plasticity is attenuated in response to exercise. Here, we report that senescent cells, identified using senescence markers senescence-associated β-Galactosidase (SA β-Gal) and p21 are very infrequent in resting muscle but emerge approximately two-weeks after a bout of resistance exercise in humans. We hypothesized that these cells contribute to blunted hypertrophic potential in old age. Using synergist ablation-induced mechanical overload of the plantaris muscle to model resistance training in adult (5-6 month) and old (23-24 month) male C57BL/6J mice, we found increased senescent cells in both age groups during hypertrophy. Consistent with the human data, there were negligible senescent cells in adult and old sham controls, but old mice had significantly more senescent cells 7- and 14-days following overload relative to young. Old mice had blunted whole muscle hypertrophy when compared to adult mice, along with smaller muscle fibers, specifically glycolytic Type 2x+2b fibers. To ablate senescent cells using a hit-and-run approach, old mice were treated with vehicle or a senolytic cocktail consisting of 5 mg/kg dasatinib and 50 mg/kg quercetin (D+Q) on day 7 and 10 during 14-days of overload; control mice underwent sham surgery with or without senolytic treatment. Old mice given D+Q had larger muscles and muscle fibers after 14 days of overload, fewer senescent cells when compared to vehicle-treated old mice, and changes in the expression of genes (i.e., Igf1, Ddit4, Mmp14) that are associated with hypertrophic growth . Our data collectively show that senescent cells emerge in human and mouse skeletal muscle following a hypertrophic stimulus, and that D+Q improves muscle growth in old mice.

2022-05-29 | GSE195707 | GEO

Coordinated Regulation of Myonuclear DNA Methylation, mRNA, and miRNA Levels Associates with the Metabolic Response to Rapid Synergist Ablation-Induced Skeletal Muscle Hypertrophy in Female Mice

Project description:Coordinated Regulation of Myonuclear DNA Methylation, mRNA, and miRNA Levels Associates with the Metabolic Response to Rapid Synergist Ablation-Induced Skeletal Muscle Hypertrophy in Female Mice

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