Determinng global implications of the ADAR3 R-domain on mediating ADAR3-RNA interactions

ABSTRACT: Although R-domain has been implicated in RNA binding in vitro, whether ADAR3 uses the arginine-rich R-domain to mediate ADAR3-RNA interactions in vivo remain to be explored. Here, an unbiased, high-throughput approach was undertaken to understand if mutating the R-domain would affect the ability of ADAR3 to either bind and/or recognize target transcripts. Although R-domain doesn’t confer target specificity to ADAR3, the R-domain mutant is associated with decreased protein stability and might reduce the ability of ADAR3 to bind target RNAs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE247886 | GEO | 2023/12/01

REPOSITORIES: GEO