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Transcriptional dynamics orchestrating the development and integration of neurons born in the adult hippocampus [ds1]

ABSTRACT: To profile neurogenesis in the adult mouse hippocampus, we utilized a strategy to isolate developing cells derived from RGLs expressing the proneural gene Achaete-scute homolog 1 (Ascl1). Tamoxifen administration to young-adult Ascl1CreERT2;CAGfloxStopSun1sfGFP mice were used to induce the expression of Sun1-sfGFP in the nuclear membrane of RGLs and permanently label their progeny. This birthdating tag enabled a comprehensive analysis of the entire process encompassing neuronal differentiation, development, and functional maturation. This dataset was built using FACS-sorted nuclei obtained from dentate gyri microdissected at 1-, 2-, 4-, and 8 weeks after TAM induction (cohorts w1 through w8)

ORGANISM(S): Mus musculus

PROVIDER: GSE249795 | GEO | 2024/07/19

REPOSITORIES: GEO

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Transcriptional dynamics orchestrating the development and integration of neurons born in the adult hippocampus [ds2]

Project description:To profile neurogenesis in the adult mouse hippocampus, we utilized a strategy to isolate developing cells derived from RGLs expressing the proneural gene Achaete-scute homolog 1 (Ascl1). Tamoxifen administration to young-adult Ascl1CreERT2;CAGfloxStopSun1sfGFP mice were used to induce the expression of Sun1-sfGFP in the nuclear membrane of RGLs and permanently label their progeny. This birthdating tag enabled a comprehensive analysis of the entire process encompassing neuronal differentiation, development, and functional maturation. This dataset was built using FACS-sorted nuclei obtained from dentate gyri microdissected at 2-, 3-, 4-, 5- and 8 weeks after TAM induction (cohorts w2 through w8)

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Expression data from an ASCL1 siRNA experiment

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Project description:Transcription factor Achaete-Scute homologue 2 initiates T follicular helper cell development [array]

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Intravital imaging identifies long-term self-renewing stem cells in the adult mouse hippocampus

Project description:Neural stem cells (NSCs) generate new neurons throughout life in the mammalian hippocampus. However, the potential for long-term self-renewal of individual NSCs within the adult brain remains unclear. We used chronic in vivo 2-photon microscopy and followed single NSCs that were genetically labeled through conditional recombination driven by the regulatory elements of the stem cell-expressed genes GLI Family Zinc Finger 1 (Gli1) or Achaete-scute homolog 1 (Ascl1). Through intravital imaging of NSCs and their progeny we identify a population of Gli1-targeted NSCs showing long-term self-renewal in the adult hippocampus. In contrast, once activated, Ascl1-targeted NSCs undergo limited proliferative activity before they becoming exhausted. Using protein expression profiling and single-cell RNA sequencing (scRNA-seq), we show that Gli1- and Ascl1-targeted cells have highly similar yet distinct transcriptional profiles, supporting the existence of heterogeneous NSC populations with diverse behavioral properties. Thus, we here provide the cellular framework for how functional diversity of NSCs enables the generation of new neurons in the adult hippocampus.

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