Transcriptomics

Dataset Information

0

The miR-183/96/182 Cluster Regulates Sensory Innervation, Resident Myeloid Cells and Functions of the Cornea Through Cell type-specific Target Genes


ABSTRACT: The miR-183/96/182 cluster (miR-183C) is specifically expressed in sensory neurons and immune cells and modulates corneal immune/inflammatory response to bacterial infection. To uncover the roles of miR-183C in corneal homeostasis through its regulation of sensory neurons of the trigeminal ganglia (TG) and innate myeloid cells, we created miR-183C conventional knockout (KO), and sensory nerve-specific (SNS-CKO) and myeloid cell-specific conditional knockout (MS-CKO) mice. We performed 3'RNA sequencing in the TG and corneas of these knockout mice and their wild type (WT)-control littermates. To study specific functions of miR-183C in corneal resident myeloid cells (CRMCs), we isolated Csf1r-EGFP+ CRMCs from conventional KO and MS-CKO and their WT control mice. By comparison of the gene expression profiles of the KO or CKO vs their corresponding WT mice, we identified a series of differentially expressed genes. Bioinformatic analyses uncovered tissue- and/or cell-type-specific target genes of miR-183C. Functional annotation of the target genes revealed functions of miR-183C in TG and CRMCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE250213 | GEO | 2023/12/20

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2019-08-17 | GSE118675 | GEO
2021-08-18 | GSE177057 | GEO
2024-01-10 | GSE249384 | GEO
2016-01-01 | GSE73594 | GEO
2015-02-21 | E-GEOD-65524 | biostudies-arrayexpress
2018-09-18 | GSE110239 | GEO
2024-08-02 | PXD049258 | Pride
2015-08-25 | GSE69804 | GEO
2022-07-19 | E-MTAB-11888 | biostudies-arrayexpress
2020-10-27 | GSE106298 | GEO