Genome-wide analysis of IFNa response in WT and STAT3KO MEFs
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE25044 | GEO | 2010/11/04
SECONDARY ACCESSION(S): PRJNA134545
REPOSITORIES: GEO
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Project description:Analysis of gene expression profiles in MEF cell lines in the absence or presence of STAT3 following IFNa stimulation. This study is to unveil possible role of STAT3 during type I IFN-mediated gene induction and functions. Total RNA obtained from WT or STAT3KO MEFs stimulated without or with IFNa 1000 U/ml for 2 h.
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Project description:We describe finely resolved kinetics of the transcriptional response to IFNa in B cells in vivo. Temporal changes in expression of the most robustly induced ISGs were analyzed across 23 (15min - 15hrs) timepoints. Most ISGs reach peak induction at approximately 2hrs and generally in a synchronus manner. These analses provide insight into the regulatory network structure of IFN responses.
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