Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiling in preadipocytes in obese Pima Indians/humans

ABSTRACT: Microarray analysis comparing gene expression profiles of primary cultured preadipocytes from non-diabetic lean vs non-diabetic obese Pima Indian subjects (a subset of the subjects from the adipocyte genechip project, GSE2508). Primary cultured abdominal subcutaneous preadipocytes from 14 lean (7 Males / 7 Females) and 14 obese (7M/7F) subjects were hybridized individually to Affymetrix oligonucleotide arrays HG-U133A and B. Keywords = Obesity Keywords = inflammation Keywords = microarray Keywords = gene expression Keywords = preadipocyte Keywords = stromal-vascular cells Keywords = adipose tissue Keywords: other

ORGANISM(S): Homo sapiens

PROVIDER: GSE2510 | GEO | 2005/07/28

SECONDARY ACCESSION(S): PRJNA92043

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of cultured human preadipocytes/stromal vascular cells from obese and non-obese Pima Indians

Project description:Microarray analysis comparing gene expression profiles of primary cultured preadipocytes from non-diabetic lean vs non-diabetic obese Pima Indian subjects (a subset of the subjects from the adipocyte genechip project, GSE2508). Primary cultured abdominal subcutaneous preadipocytes from 14 lean (7 Males / 7 Females) and 14 obese (7M/7F) subjects were hybridized individually to Affymetrix oligonucleotide arrays HG-U133A and B.

2007-08-25 | E-GEOD-2510 | biostudies-arrayexpress

Transcription profiling of human abdominal subcutaneous adipocytes from obese and non-obese Pima Indians

Project description:Microarray analysis comparing gene expression profiles of adipocytes from non-diabetic lean vs non-diabetic obese Pima Indian subjects to identify differentially expressed adipocyte genes with obesity. RNA samples of isolated abdominal subcutaneous adipocytes from 20 lean (10 Males / 10 Females, aged 31Â±6 year, Body Mass Index 25Â±3 kg/m2 ) and 19 obese (9M/10F, 29Â±5y, 55Â±8 kg/m2 ) subjects were hybridized individually to Affymetrix oligonucleotide arrays HG-U95A, B, C, D, and E.

2007-08-25 | E-GEOD-2508 | biostudies-arrayexpress

Expression profiling in adipocytes of obese humans

Project description:Microarray analysis comparing gene expression profiles of adipocytes from non-diabetic lean vs non-diabetic obese Pima Indian subjects to identify differentially expressed adipocyte genes with obesity. RNA samples of isolated abdominal subcutaneous adipocytes from 20 lean (10 Males / 10 Females, aged 31±6 year, Body Mass Index 25±3 kg/m2 ) and 19 obese (9M/10F, 29±5y, 55±8 kg/m2 ) subjects were hybridized individually to Affymetrix oligonucleotide arrays HG-U95A, B, C, D, and E. Keywords = adipocyte Keywords = obesity Keywords = inflammation Keywords = gene expression Keywords: other

2006-01-01 | GSE2508 | GEO

Type 2 diabetes and insulin resistance

Project description:Global transcript profiling to identify differentially expressed skeletal muscle genes in insulin resistance, a major risk factor for Type II (non-insulin-dependent) diabetes mellitus. Compared gene expression profiles of skeletal muscle tissues from 18 insulin-sensitive versus 17 insulin-resistant equally obese, non-diabetic Pima Indians. Keywords: other

2002-12-17 | GSE121 | GEO

Characterization of the human preadipoyte proteome

Project description:Identification of proteome of preadipocytes isolated from morbidly obese subjects

2022-02-15 | PXD015621 | Pride

Adipocyte-Derived Exosomal microRNAs in subject serum samples

Project description:Affymetrix miRNA 4.0 array profiling of adipocyte-derived exosomes from lean and obese subjects. We used miRNA arrays to profile FABP4+ (adipocyte-derived) exosomes isolated from serum of lean and obese subjects.

2019-06-03 | GSE125494 | GEO

Homo sapiens

Project description:Expression profiling in preadipocytes in obese Pima Indians/humans

| PRJNA92043 | ENA

Subcutaneous adipose tissue from lean and obese subjects

Project description:Obtaining adipose tissue samples are paramount to the understanding of human obesity. We have examined the impact of needle-aspirated and surgical biopsy techniques on the study of subcutaneous adipose tissue (scAT) gene expression in both obese and lean subjects. Biopsy sampling methods have a significant impact on data interpretation and revealed that gene expression profiles derived from surgical tissue biopsies better capture the significant changes in molecular pathways associated with obesity. We hypothesize that this is because needle biopsies do not aspirate the fibrotic fraction of scAT; which subsequently results in an under-representation of the inflammatory and metabolic changes that coincide with obesity. This analysis revealed that the biopsy technique influences the gene expression underlying the biological themes commonly discussed in obesity (e.g. inflammation, extracellular matrix, metabolism, etc), and is therefore a caveat to consider when designing microarray experiments. These results have crucial implications for the clinical and physiopathological understanding of human obesity and therapeutic approaches. Keywords: subject and tissue biopsy technique comparison Tissue samples from lean and obese subjects were analyzed: total of 36 hybridizations. The goal was to compare the effect of biopsy sampling methods on global subcutaneous adipose tissue gene expression analyses. The following subject groups were used for the analysis: 9 lean subjects: needle biopsy 9 lean subjects: surgical biopsy 9 obese subjects: needle biopsy 9 obese subjects: surgical biopsy

2008-12-22 | E-GEOD-12050 | biostudies-arrayexpress

Adipocyte-derived Exosomal miRNAs from Subcutaneous Adipose Tissue: A Novel Mechanism for Obesity-Related Disease

Project description:Affymetrix miRNA 3.0 array profiling of adipocyte-derived exosomes from obese and lean human subjects. We used miRNA arrays to profile exosomes shed from obese and lean human subcutaneous fat that was cultured for 60 minutes.

2017-05-15 | GSE68885 | GEO

Adipocyte-derived Exosomal miRNAs from Visceral Adipose Tissue: A Novel Mechanism for Obesity-Related Disease

2015-03-03 | GSE50574 | GEO