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Transcription profiling of cultured human preadipocytes/stromal vascular cells from obese and non-obese Pima Indians

ABSTRACT: Microarray analysis comparing gene expression profiles of primary cultured preadipocytes from non-diabetic lean vs non-diabetic obese Pima Indian subjects (a subset of the subjects from the adipocyte genechip project, GSE2508). Primary cultured abdominal subcutaneous preadipocytes from 14 lean (7 Males / 7 Females) and 14 obese (7M/7F) subjects were hybridized individually to Affymetrix oligonucleotide arrays HG-U133A and B.

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Saraswathy Nair

PROVIDER: E-GEOD-2510 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Increased expression of inflammation-related genes in cultured preadipocytes/stromal vascular cells from obese compared with non-obese Pima Indians.

Nair S S Lee Y H YH Rousseau E E Cam M M Tataranni P A PA Baier L J LJ Bogardus C C Permana P A PA

Diabetologia 20050721 9

<h4>Aims/hypothesis</h4>The specific contributions made by the various cell types in adipose tissue to obesity, particularly obesity-related inflammation, need to be clarified. The aim of this study was to elucidate the potential role of adipocyte precursor cells (preadipocytes/stromal vascular cells [SVC]).<h4>Methods</h4>We performed Affymetrix oligonucleotide microarray expression profiling of cultured abdominal subcutaneous preadipocytes/SVC isolated from the adipose tissue of 14 non-obese ( ...[more]

PMID: 16034612

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