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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human abdominal subcutaneous adipocytes from obese and non-obese Pima Indians

ABSTRACT: Microarray analysis comparing gene expression profiles of adipocytes from non-diabetic lean vs non-diabetic obese Pima Indian subjects to identify differentially expressed adipocyte genes with obesity. RNA samples of isolated abdominal subcutaneous adipocytes from 20 lean (10 Males / 10 Females, aged 31Â±6 year, Body Mass Index 25Â±3 kg/m2 ) and 19 obese (9M/10F, 29Â±5y, 55Â±8 kg/m2 ) subjects were hybridized individually to Affymetrix oligonucleotide arrays HG-U95A, B, C, D, and E.

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Yong-Ho Lee

PROVIDER: E-GEOD-2508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray profiling of isolated abdominal subcutaneous adipocytes from obese vs non-obese Pima Indians: increased expression of inflammation-related genes.

Lee Y H YH Nair S S Rousseau E E Allison D B DB Page G P GP Tataranni P A PA Bogardus C C Permana P A PA

Diabetologia 20050730 9

<h4>Aims/hypothesis</h4>Obesity increases the risk of developing major diseases such as diabetes and cardiovascular disease. Adipose tissue, particularly adipocytes, may play a major role in the development of obesity and its comorbidities. The aim of this study was to characterise, in adipocytes from obese people, the most differentially expressed genes that might be relevant to the development of obesity.<h4>Methods</h4>We carried out microarray gene profiling of isolated abdominal subcutaneou ...[more]

PMID: 16059715

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