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AML/T cell interactomics uncover correlates of patient outcomes and the key role of ICAM1 in T cell killing of AML

ABSTRACT: T cells are important for the control of acute myeloid leukemia (AML), a common and often deadly malignancy. We observed that some AML patient samples are resistant to killing by human engineered cytotoxic CD4+ T cells. Single-cell RNA-seq of primary AML samples and CD4+ T cells before and after their interaction uncovered transcriptional programs that correlate with AML sensitivity or resistance to CD4+ T cell killing. Resistance-associated AML programs were enriched in AML patients with poor survival, and killing-resistant AML cells did not engage T cells in vitro. Killing-sensitive AML potently activated T cells before being killed, and upregulated ICAM1, a key component of the immune synapse with T cells. Without ICAM1, killing-sensitive AML became resistant to killing to primary ex vivo-isolated CD8+ T cells in vitro, and engineered CD4+ T cells in vitro and in vivo. Thus, ICAM1 on AML acts as an immune trigger, allowing T cell killing, and could affect AML patient survival in vivo.

ORGANISM(S): Homo sapiens

PROVIDER: GSE254282 | GEO | 2024/05/09

REPOSITORIES: GEO

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AML/T cell interactomics uncover correlates of patient outcomes and the key role of ICAM1 in T cell killing of AML

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