Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome-wide mapping of RNA:protein interactions of Arid5a in mouse P19 cells.

ABSTRACT: We performed Arid5a iCLIP2 in P19 cells transiently expressing GFP-tagged Arid5a.

ORGANISM(S): Mus musculus

PROVIDER: GSE254818 | GEO | 2024/08/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome-wide mapping of RNA:protein interactions of SRSF5 in HeLa cells

Project description:We performed SRSF5 iCLIP2 in HeLa cells expressing endogenously GFP-tagged SRSF5.

2023-12-11 | GSE229325 | GEO

Transcriptome-wide mapping of RNA:protein interactions of GFP-RS2Z35 and GFP-RS2Z36 in tomato (Solanum lycopersicum) leaves.

Project description:We performed iCLIP2 in tomato leaves stably expressing GFP-tagged RS2Z35 or RS2Z36.

2024-08-25 | GSE260668 | GEO

iCLIP mapping of coilin-RNA interactions in HeLa and P19 cells

Project description:Our ChIP resuls suggested that coilin association with U3, snRNA and histone genes might be dependent on coilin-RNA interaction. We used iCLIP of coilin-GFP expressed in HeLa and P19 cell lines at endogenous levels to identify coilin RNA targets and investigate RNA-binding specificity. P19 cells expressing GFP fused to a nuclear localization signal (GFP-NLS) was used as a negative control. iCLIP results revealed that coilin binds several classes of ncRNA including snRNAs, U3 snoRNA and scaRNAs. Interestlignly the majority of coilin targets were intronic snoRNAs, suggesting a novel role of CBs in snoRNA biogenesis. 5 biological replicates from P19 and 2 biological replicates from HeLa cells after UV-crosslinking. Negative control samples prepared from GFP-NLS fusion protein are stored uder accession E-MTAB-747.

2014-11-06 | E-GEOD-58448 | biostudies-arrayexpress

Transcriptome-wide mapping of RNA:protein interactions of SRSF7 variants in P19 cells by iCLIP and piCLIP

Project description:We used P19 cells that overexpress GFP-tagged SRSF7 by 3.4-fold and expression-matched SRSF7 mutants that either lack a functional Zinc-knuckle domain or a part of their RS-domain and compared the extent and pattern of binding to mRNAs. For this we performed iCLIP using anti-GFP antibodies. We also performed iCLIP from monosomal and polysomal fractions. In addition we performed iCLIP using anti-SRSF7 antibodies to compare the binding patterns of SRSF7-GFP, endogenous SRSF7 protein and its truncated SRSF7_RRM variant.

2020-03-02 | GSE140721 | GEO

iCLIP mapping of coilin-RNA interactions in HeLa and P19 cells

2014-11-06 | GSE58448 | GEO

RNA-Seq and Ribo-Seq of P19 WT and SRSF7-GFP overexpressing cells

Project description:We used P19 cells that overexpress GFP-tagged SRSF7 by 3.4-fold.

2020-03-02 | GSE142800 | GEO

Peptide mapping of SRSF7_RS-GFP isoforms in murine P19 cells

Project description:Here we mapped peptides of mouse SRSF7_RS-GFP purified from P19 cells.

2020-05-27 | PXD016871 | Pride

Differential expression of mature miRNAs in P19 cells during neuronal differentiation and by Rbfox3 depletion.

2014-09-21 | GSE52377 | GEO

Differential expression of mature miRNAs in P19 cells during neuronal differentiation and by Rbfox3 depletion.

Project description:To investigate whether Rbfox3 could alter the expression level of miRNAs during neuronal differentiation of P19 cells, we performed miRNA microarray analysis using the RNAs extracted from untreated (undifferentiated) P19-GFP, RA-treated (neuronally differentiated) P19-GFP, or RA-treated P19-T2 cells. Total 9 samples were analyzed. We compared expression levels of P19-GFP (-) vs P19-GFP (+) vs P19-T2 (+) to identify miRNAs which had changes in expression levels with p < 0.01. From this miRNA list, we compared among P19-GFP (-) vs P19-GFP (+) vs P19-T2 (+) to identify the miRNAs which appeared to correlate with Rbfox3 expression.

2014-09-21 | E-GEOD-52377 | biostudies-arrayexpress

Peptide mapping of SRSF7_RRM isoforms in murine P19 cells

Project description:Here we mapped peptides of mouse SRSF7_RRM purified from P19 cells.

2020-05-27 | PXD016884 | Pride