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Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [dorsal root ganglia]

ABSTRACT: We applied Solexa sequencing technology to identify rat microRNA genes in dorsal root ganglia (DRGs) following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 114 novel miRNAs in rats were discovered and identified, of which 52 novel miRNAs were first reported in rat DRGs and 62 novel miRNAs were produced at days 1, 4, 7 and 14 after sciatic nerve resection. These data provide an important resource relating to the role and regulation of miRNAs for future studies relating to peripheral nerve injury and regeneration.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE25863 | GEO | 2012/05/06

SECONDARY ACCESSION(S): PRJNA135789

REPOSITORIES: GEO

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Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [dorsal root ganglia]

Project description:We applied Solexa sequencing technology to identify rat microRNA genes in dorsal root ganglia (DRGs) following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 114 novel miRNAs in rats were discovered and identified, of which 52 novel miRNAs were first reported in rat DRGs and 62 novel miRNAs were produced at days 1, 4, 7 and 14 after sciatic nerve resection. These data provide an important resource relating to the role and regulation of miRNAs for future studies relating to peripheral nerve injury and regeneration. 18-30 nt small RNAs from 30 Thirty Sprague-Dawley (SD) rats were sequenced at one Solexa lane

2012-05-06 | E-GEOD-25863 | biostudies-arrayexpress

Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [proximal sciatic nerve]

Project description:We applied Solexa sequencing technology to identify rat microRNA genes in proximal sciatic nerve following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 93 novel miRNAs in rats were discovered and identified, of which 42 novel miRNAs were first reported in proximal sciatic nerve of rat and 51 novel miRNAs were produced at days 1, 4, 7 and 14 after sciatic nerve resection. These data provide an important resource relating to the role and regulation of miRNAs for future studies relating to peripheral nerve injury and regeneration. Keywords: Small RNA sequencing

2012-05-06 | GSE26753 | GEO

Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [proximal sciatic nerve]

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2010-06-12 | E-GEOD-22181 | biostudies-arrayexpress

Entrapment Neuropathy Results in Different MicroRNAs Expression Patterns from Denervation Injury in Rats

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Altered miRNA expression of cultured dorsal root ganglia neurons at different times of rat

Project description:To explored the function of miRNAs during the axon regeneration of rat dorsal root ganglia neurons, we utilized miRCURYTM LNA Array (v.18.0) to find out the altered expression of miRNAs in cultured DRG neurons at different times. When we digest the cells from rat DRGs with enzymes, we only get the cell bodies without axons. During the axons re-growth after the neurons planted, it must be amount of gene and miRNAs will changed. However, we still not fully understand which miRNAs and mRNA will changed and the function of these miRNAs and mRNAs. In addition, this cell model could mimic the regeration of sciatic nerve after injure in vivo, which is still a challenge in clinical.

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