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RIP-seq reveals the interactome of nucleolin in activated CD4+ T cells

ABSTRACT: During the actiation of CD4+T cells, the pri-miRNAs translocated from cytoplasma into nucleus.However, the mechanism remains unkown. We found that pri-miR-31 interact with nucleolin through G-guadruplex struction in pri-miR-31. We then transfected ASO to destribute the interaction of nucleolin and G4 struction. In order to identify the interacting RNA, including miRNA precuser and mRNA, we performed the RIP-seq Immunoprecipited by anti-nucleolin and IgG.

ORGANISM(S): Mus musculus

PROVIDER: GSE263822 | GEO | 2025/04/11

REPOSITORIES: GEO

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