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Multi-omics integration reveals the dynamic impacts of stock enhancement on the intestinal health condition in kaluga sturgeon (Huso dauricus)

ABSTRACT: On October 6th,2023, one thousand kaluga sturgeon were released to Songhua River (119°52′-132°31′ E,11°12′-51°38′ N) after 2 h adaptive domestication to eliminate the physiological response caused by transport stress. Before wild reintroduction to river, non-toxic fluorescent dye was injected into the snout of kaluga sturgeon, which was beneficial to catch them again. On the 0 d (Ori), 7 d (D7FL), 14 d(D14FL) and 30 d (D30FL), three kaluga sturgeon individuals were recaptured randomly using seine nets. We performed a series of experiment to assess the dynamic effects of stock enhancement on the gut health conditions.

ORGANISM(S): Huso dauricus

PROVIDER: GSE266327 | GEO | 2024/05/01

REPOSITORIES: GEO

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Project description:The biology and physiology of Siberian sturgeon reproduction differ substantially from teleost fish. Seminal plasma support and protect the viability, motility and fertilizing capacity of spermatozoa by creating the optimal environment for the storage. The present study for the first time described in-depth proteomic characterization of Siberian sturgeon seminal plasma using gel based and gel-free proteomic approaches: LC-MS/MS, 2DE and 2D blue native (BN)/SDS-PAGE. LC-MS/MS allowed to identify 657 proteins, which were classified according to their functions and pathways. 2DE visualized 339 spots corresponding to 76 unique proteins (almost 60% were present in various proteoforms). For the first time we demonstrated interactions between seminal proteins; four (C1-C4) multiprotein complexes were identified after 2D BN/SDS-PAGE; composed of (C1) serotransferrin-2 (TF) and fish-egg lectin (FEL); (C2) serum albumin 2 (ALB) and retinol-binding protein 4 (RBP4); (C3) ALB and TF (C3) and (C4) apolipoprotein A-I (APOA1), riboflavin-binding protein (RfBP) and myoglobin (MB). BN-SDS-PAGE also revealed that immunoglobulin and TF formed homomultimeric (dimer, trimer, tetramer and pentamer) complexes in seminal plasma. ALB, TF, Beta-Ala-His dipeptidase, glyceraldehyde-3-phosphate dehydrogenase, IGH, APOA1, hemopexin, type-4 ice-structuring protein, FEL, RfBP and glycogen phosphorylase, liver form-like were selected as major seminal plasma proteins. The functional analysis of identified proteins indicated their involvement mainly in immune system process and response to stimulus, metabolism, vesicle-mediated transport, proteolysis, and catherin binding involved in cell-cell adhesion. Moreover, 67 proteins were associated with reproductive processes, including spermatogenesis, fertilization, acrosomal reaction and sperm motility. Comparative proteomic analysis of sturgeon seminal plasma showed common proteins with fish and human and 150 proteins specific for sturgeon which may reflect the specificity of sturgeon reproduction. To conclude, this integrative proteomic view lead to deeper insight into physiological function of seminal plasma and processes occurred in sturgeon reproductive tract.

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Multi-omics integration reveals the dynamic impacts of stock enhancement on the intestinal health condition in kaluga sturgeon (Huso dauricus)

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