Transcriptomics

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Total RNA-seq and long-read RNAseq (Nanopore) upon Son KD in mouse embryonic stem cells


ABSTRACT: Gene-expression noise can influence cell-fate choices across pathology and physiology. However, a crucial question persists: do regulatory proteins or pathways exist that control noise independently of mean expression levels? Resulting from a previous screen, the protein SON was identified as a potential noise regulator. We perform Son KD and utilize (1) total RNA sequencing (RNA-seq) to analyze differences in splicing efficiency of introns upon Son KD; and (2) Nanopore sequencing to analyze changes in isoform usage. These datasets correspond to the aforementioned total RNA-seq and long-read polyA+ cDNA Nanopore sequencing upon Son KD.

ORGANISM(S): Mus musculus

PROVIDER: GSE271659 | GEO | 2024/08/05

REPOSITORIES: GEO

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