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ATAC-Seq Analysis Reveals Arid3a-Dependent Chromatin Accessibility Changes in VSMCs

ABSTRACT: This study leverages ATAC-seq to reveal Arid3a's chromatin accessibility effects in VSMCs, identifying key gene expression changes and implicating Arid3a in actin filament dynamics linked to AAA pathogenesis.

ORGANISM(S): Mus musculus

PROVIDER: GSE275617 | GEO | 2024/08/30

REPOSITORIES: GEO

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Epigenetic Regulation of VSMCs by Arid3a: Insights from RNA-seq Analyses

Project description:This study employs RNA-seq to investigate the transcriptional effects of Arid3a in Vascular Smooth Muscle Cells (VSMCs), revealing a comprehensive signature of gene expression. We identified 394 genes that were downregulated and 227 that were upregulated in response to Arid3a knockdown, shedding light on its regulatory network. The RNA-seq data highlighted the key role of Arid3a in controlling the dynamics of actin filaments, which has implications for processes such as cell adhesion and the cAMP signaling pathway.

2024-08-30 | GSE275618 | GEO

CUT&Tag Profiling Reveals Arid3a-Dependent Epigenetic Control in Vascular Smooth Muscle Cells

Project description:This study employs CUT&Tag technology to meticulously map the chromatin-binding landscape of the transcription factor Arid3a in Vascular Smooth Muscle Cells (VSMCs), thereby highlighting its crucial function in the intricate regulation of gene expression. The CUT&Tag assay illuminates Arid3a's presence at key promoter regions, exerting a profound impact on transcriptional modulation and suggesting the epigenetic governance of 260 genes. By integrating RNA-seq with sophisticated bioinformatic analyses, our research elucidates Arid3a's integral role in fundamental cellular activities, particularly in the dynamics of actin filaments, as well as its potential regulatory nexus with critical pathways, including cAMP signaling and calcium signaling. These insights unveil the intricate molecular choreography of Arid3a in the functioning of VSMCs and shed new light on its role in the development of abdominal aortic aneurysm (AAA).

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ATAC-Seq Analysis Reveals Arid3a-Dependent Chromatin Accessibility Changes in VSMCs

Project description:ATAC-Seq Analysis Reveals Arid3a-Dependent Chromatin Accessibility Changes in VSMCs

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Epigenetic Regulation of VSMCs by Arid3a: Insights from RNA-seq Analyses

Project description:Epigenetic Regulation of VSMCs by Arid3a: Insights from RNA-seq Analyses

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CMK LC-MS/MS after CoIP of ARID3A

Project description:In order to determine proteins interacting with ARID3A in the ML-DS cell line CMK, we performed CoIP of endogenous ARID3A followed by LC-MS/MS

2023-03-11 | PXD025027 | Pride

Arid3a is required for mammalian placenta development

Project description:Previous studies in the mouse indicated that Arid3a plays a critical role in the first cell fate decision required for generation of trophectoderm (TE). Here, we demonstrate that Arid3a is widely expressed during mouse and human placentation and essential for early embryonic viability. Arid3a is located within trophoblast giant cells and other trophoblast-derived cell subtypes in the junctional and labyrinth zones of the placenta. Conventional Arid3a knockout embryos suffer restricted intrauterine growth with sever defects in placental structural organization. Arid3a null placentas show aberrant expression of subtype-specific markers as well as significant alteration in inflammatory response-related genes, cytokines and chemokines. We provide evidence that BMP4-mediated induction of trophoblast stem (TS)-like cells from human induced pluripotent (iPS) stem cells results in ARID3A upregulation and cytoplasmic to nuclear translocation. Overexpression of ARID3A in human iPS and BMP4-mediated TS-like cells up-regulated TE markers, whereas pluripotent markers were down-regulated. Our results indicate that the roles of Arid3a are conserved and essential for mammalian placental development through regulation of both intrinsic and extrinsic developmental programs. Placentas of E10.5 and E11.5 wild type (WT) and Arid3a-/- mice were generated by deep sequencing, using Illumina

2016-07-01 | E-GEOD-79638 | biostudies-arrayexpress

Arid3a is required for mammalian placenta development

2016-07-01 | GSE79638 | GEO

Analysis of ARID3A regulated genes in K562 cells

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2023-06-01 | E-MTAB-12603 | biostudies-arrayexpress

Phenotype modulation of VSMCs on patterned substrate

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ARID3a Gene Profiles are Strongly Associated with Human Interferon Alpha Production

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