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CUT&Tag Profiling Reveals Arid3a-Dependent Epigenetic Control in Vascular Smooth Muscle Cells

ABSTRACT: This study employs CUT&Tag technology to meticulously map the chromatin-binding landscape of the transcription factor Arid3a in Vascular Smooth Muscle Cells (VSMCs), thereby highlighting its crucial function in the intricate regulation of gene expression. The CUT&Tag assay illuminates Arid3a's presence at key promoter regions, exerting a profound impact on transcriptional modulation and suggesting the epigenetic governance of 260 genes. By integrating RNA-seq with sophisticated bioinformatic analyses, our research elucidates Arid3a's integral role in fundamental cellular activities, particularly in the dynamics of actin filaments, as well as its potential regulatory nexus with critical pathways, including cAMP signaling and calcium signaling. These insights unveil the intricate molecular choreography of Arid3a in the functioning of VSMCs and shed new light on its role in the development of abdominal aortic aneurysm (AAA).

ORGANISM(S): Mus musculus

PROVIDER: GSE275619 | GEO | 2024/08/30

REPOSITORIES: GEO

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