Dataset Information

Transcriptomic analysis of early stages of ‘Candidatus Liberibacter asiaticus’ infection in susceptible and resistant genotypes after inoculation by Diaphorina citri feeding on young shoots

ABSTRACT: Huanglongbing (HLB) is a devastating disease of citrus plants caused by the non-culturable phloem-inhabiting bacterium Candidatus Liberibacter asiaticus (CLas). Bacteria are vectored by the psyllid Diaphorina citri and introduced into sieve cells, evading plant immunity and establishing a successful infection in citrus plants. Although no resistance has been reported in Citrus species, complete or partial resistance has been documented in the distant relatives Murraya paniculate and Bergera koenigii, providing excellent systems to investigate molecular mechanisms leading to either resistance or susceptibility. The first weeks after bacterial release into the phloem are critical for the establishment of the bacteria. In this study, a thorough transcriptomic analysis of young flushes exposed to CLas-positive or negative psyllid has been performed in Citrus x sinensis, as well as in these resistant species along the first eight weeks after exposure. Our results suggest that the resistant genotypes do not deploy a classical immunity response. Instead, transcriptome changes are scarce and only a few genes are differentially expressed. Functional analysis suggest that primary metabolism and other basic cellular functions could be rewired in the resistant genotypes to limit infection. Transcriptomes of young flushes of the three species are very different, supporting the existence of distinct biochemical landscapes. These findings suggest that both intrinsic metabolic inadequacies to CLas survival, as well as inducible reprogramming of physiological functions upon CLas recognition, could orchestrate together to restrict bacteria multiplication in these resistant hosts.

ORGANISM(S): Murraya paniculata Citrus sinensis Murraya koenigii

PROVIDER: GSE277583 | GEO | 2025/02/14

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:The Asian citrus psyllid (Diaphorina citri) is a pest of citrus and the primary insect vector of the bacterial pathogen, ‘Candidatus Liberibacter asiaticus’ (CLas), which is associated with citrus greening disease. Variability in CLas titer in insects collected from infected plants has been attributed in part to the host plant from which the insects were collected. CLas accumulates to high titers in infected Citrus macrophylla, and in D. citri feeding on the infected plants of this species. In contrast, in the citrus relative Murraya paniculata, CLas titers remain low in infected plants and in D. citri exposed to infected plants. In this study, top-down and bottom-up proteomics methods were used to investigate the impact of these different host plants on D. citri protein expression. Difference in gel electrophoresis (DIGE) was used to identify protein spots on two-dimensional gels that were larger in one of three insect sample classes compared to the other two: D. citri continuously reared on C. macrophylla, D. citri reared continuously on M. paniculata, and D. citri transferred to M. paniculata for five days feeding after continuous rearing on C. macrophylla. Peptide mass spectrometry was used to identify and quantify proteins in target spots upregulated in each sample class. Shotgun proteomics was used to identify and quantify proteins from analysis of tryptic peptide samples prepared from whole insects from four sample classes: the reciprocal host switch condition (D. citri transferred to C. macrophylla for five days feeding after continuous rearing on M. paniculata) in addition to the three sample classes used in DIGE analysis. Integration of the results of both analyses reveals proteins identified by separate experimental workflows to be upregulated in insects adapted to each host plant, and in insects adapting to a novel host plant. A peptidoglycan-degrading protein involved in the immune response to bacterial pathogens was found to be upregulated in M. paniculata-reared D. citri. In the absence of CLas infection, host plant factors specific to M. paniculata may prime the antibacterial immune response in D. citri. Understanding the insect proteins involved in the adaptation of D. citri to host plants with variation in their susceptibility to CLas will inform the development of control strategies aimed at stopping the spread of citrus greening disease.

Dataset Information

Transcriptomic analysis of early stages of ‘Candidatus Liberibacter asiaticus’ infection in susceptible and resistant genotypes after inoculation by Diaphorina citri feeding on young shoots

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets