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Comprehensive gene expression analysis of human embryonic stem cell during differentiation into neural cells

ABSTRACT: In this research we have done a comprehensive transcripteome analysis of hESC differentiation at three different stages: early neural differentiation, neural ectoderm, and mature neurons. We detected and validated time-dependent gene expression patterns and represented that the gene expression patterns exhibit early ESC differentiation. Pathway analysis revealed dynamic expression patterns of members of several signaling pathways.

ORGANISM(S): Homo sapiens

PROVIDER: GSE28633 | GEO | 2011/04/15

SECONDARY ACCESSION(S): PRJNA138883

REPOSITORIES: GEO

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Comprehensive gene expression analysis of human embryonic stem cell during differentiation into neural cells

Project description:In this research we have done a comprehensive transcripteome analysis of hESC differentiation at three different stages: early neural differentiation, neural ectoderm, and mature neurons. We detected and validated time-dependent gene expression patterns and represented that the gene expression patterns exhibit early ESC differentiation. Pathway analysis revealed dynamic expression patterns of members of several signaling pathways. The hESC cells were passaged every 7 days using collagenase/dispase and cultured under feeder-free culture conditions on Matrigel in hESC medium. Three biological replicates per time point served as the input to generate biotin-labeled cRNA using a linear amplification kit.

2011-04-15 | E-GEOD-28633 | biostudies-arrayexpress

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Project description:Human embryonic stem cells (hESCs) have the pluripotency to form any cell type in the body, making them attractive cell sources in drug screening, regenerative medicine, disease and developmental processes modeling. However, not all hESC lines have equal potency to generate desired cell types in vitro, significant variation has been observed for the differentiation efficiency of various human ESC lines, and the precise underpinning molecular mechanism are still unclear. In this work, we compared transcriptome variation of four hESC lines H7, HUES1, HUES8 and HUES9 to find out whether gene expression difference between hESC lines influence their differentiation behavior in vitro. Results demonstrate that different expression genes (DEGs) are significantly enriched in developmental process, such as ectoderm, mesoderm and endoderm development, and the enrichment difference between lines is in accordance with its lineage bias. Among these DEGs, pluripotency factors and genes involved in signaling transduction also show great variation among lines. The Pleiotropic functional role of these genes in hESC identity controlling and early lineage specification, implicate that different hESC lines with distinct differentiation propensity may utilize individual balance mechanism to maintain pluripotent state, whereas the balance broken in a certain environment, gene expression variation between them could impact on their different lineage specification behavior.

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Homo sapiens

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