Arabidopsis: Control- vs. acetic acid treatment
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE29259 | GEO | 2011/12/30
SECONDARY ACCESSION(S): PRJNA140261
REPOSITORIES: GEO
Similar Datasets
Project description:Comparing Arabidopsis plants gene expresion in normal conditions (control) with acetic acid treated plants (acetic). Plants were grown on liquid MS media for 13 days, then they were transfered to MS liquid media (control) or MS+3,5 acetic acid (acetic) for two hours. Two-condition experiment, Control vs Acetic. Biological replicates: 4 control, 4 treated with acetic, independently grown. One replicate per array.
2011-12-30 | E-GEOD-29259 | biostudies-arrayexpress
Project description:External application of acetic acid has been recently reported to enhance the survival to drought in plants such as Arabidopsis, rapeseed, maize, rice and wheat, but the effects of acetic acid application on increased drought tolerance in woody plants such as a tropical crop “cassava” remain elusive. A molecular understanding of acetic acid-induced drought avoidance in cassava will contribute to the development of technology that can be used to enhance drought tolerance without resorting to transgenic technology or advancements in cassava cultivation. In the present study, morphological, physiological and molecular responses to drought were analyzed in cassava after the treatment with acetic acid. Results indicated that the acetic acid-treated cassava plants had a higher level of drought avoidance than water-treated, control plants. Specifically, higher leaf relative water content, and chlorophyll and carotenoid levels were observed as soils dried out during the drought treatment. Leaf temperatures in acetic acid-treated cassava plants were higher relative to leaves on plants pretreated with water and the increase of ABA content was observed in leaves of acetic acid-treated plants, suggesting that stomatal conductance and the transpiration rate in leaves of acetic acid-treated plants decreased to maintain relative water contents and avoid drought. Transcriptome analysis revealed that the acetic acid treatment increased the expression of ABA signaling-related genes, such as OPEN STOMATA 1 (OST1) and protein phosphatase 2C; as well as drought response and tolerance-related genes, such as outer membrane tryptophan-rich sensory protein (TSPO), and heat shock proteins. Collectively, the external application of acetic acid enhances drought avoidance in cassava through the upregulation of ABA signaling pathway genes and several stress response- and tolerance-related genes. These data support the idea that adjustments of the acetic acid application to plants is useful to enhance drought tolerance in order to minimize the growth inhibition in the agricultural field.
2019-05-29 | GSE122140 | GEO
Project description:To understand the role of acetic acids in drought tolerance, we have employed transcriptional profiling of plants treated with (or without) acetic acid and subsequent drought treatments. Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) were used.
2017-04-29 | GSE46524 | GEO
Project description:Acetic Acid Bacteria and Cellulose
| PRJEB28689 | ENA
Project description:To understand the role of acetic acids in drought tolerance, we have employed transcriptional profiling of plants treated with (or without) acetic acid and subsequent drought treatments. Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) were used. Arabidopsis Col-0 were grown in soil for 2 weeks (16 hours light / 8 hours dark). 10mM acetic acid was supplied for 9 days. For drought-treated samples, the plants were subjected to drought by withholding water supply for indicated days. Then total RNA was prepared from the aboveground tissue and used for the microarray hybridization. Three replicative hybridization experiments for each array were carried out using the independent biological samples.
2017-04-29 | E-GEOD-46524 | biostudies-arrayexpress
Project description:Despite the general acceptance of formic acid as the additive of choice for peptide reversed-phase LC-MS/MS applications, some still argue that the selection of acetic acid represents a better option. To settle this debate, we investigated both the difference in MS sensitivity and chromatographic behaviour of peptides between these two systems. This interlaboratory study was performed using different MS setups and C18 separation media employing both 0.1% formic and 0.5% acetic acid as ion pairing modifier. Relative to formic acid, we find an overall ~2.2-2.5x increase in MS signal and slight decrease in RP LC retention (-0.7% acetonitrile on average) for acetic acid conditions. While these two features have opposing effects on peptide detectability, we find that acetic acid produces up to 60% higher peptide ID output depending on the type of sample. The drop in RPLC retention increases with peptide net charge at acidic pH. MS signal is dependent on the difference between charge of the precursor ion and charge of peptide in solution, favoring species with low pI. Lower peptide retention under acetic acid conditions demonstrates its higher hydrophilicity and as expected, leads to composition and sequence-dependent character of the observed retention shift. A custom version of SSRCalc retention prediction model has been developed to accommodate these charges, with formic and acetic acid models showing identical results independent of the origin of data collection.
2022-12-10 | PXD029416 | JPOST Repository
Project description:Approximately 500 surface sterilized seeds of Arabidopsis seeds (ecotype: Col-0) were sterilely sown on filter disks overlaying solid ½ MS media 1% sucrose, stratified at 4C for 48 h and grown in darkness at 25C for 4 d. Seedlings were gently submerged by application to the plates of the different auxin solutions (made in ethanol carrier; 0.1% final concentration) and at the indicated times (20 min, 40 min, 60 min), the seedlings were lifted from the plate en mass and flash frozen in liquid nitrogen. Keywords: time-course
2003-10-12 | GSE664 | GEO
Project description:Genes whose expression correlated to the acetic acid tolerance in S. cerevisiae were identified by DNA microarray analysis. Gene expression profiles of two S. cerevisiae strains showing different levels of acetic acid tolerance were compared and an acetic acid tolerance-related gene chosen.
2012-08-30 | E-GEOD-36914 | biostudies-arrayexpress
Project description:Nitrogen (N) and phosphorus (P) are the most limiting factors for plant growth. Some microorganisms improve the uptake and availability of N and P, minimizing chemical fertilizer dependence. It has been published that the RD64 strain, a Sinorhizobium meliloti 1021 strain engineered to overproduce indole-3-acetic acid (IAA), showed improved nitrogen fixation ability as compared to the wild type 1021 strain. We present here data showing that RD64 is also highly effective in mobilizing P from insoluble sources such as phosphate rock (PR). Under P-limiting conditions, the higher P-mobilizing activity of RD64, as compared to the 1021 wild type strain, is connected with the up-regulation of genes coding for the high-affinity P transport system, the induction of acid phosphatase activity and the increased secretion into the growth media of malic, succinic and fumaric acids. Medicago truncatula plants nodulated by RD64 (Mt-RD64), when grown under P-deficient conditions, released higher amounts of another P-solubilizing organic acid, the 2-hydroxyglutaric acid, as compared to the plants nodulated by the wild-type strain (Mt-1021). It has already been shown that Mt-RD64 plants exhibited a higher dry weight production as compared to Mt-1021 plants. Here we report that also P-starved Mt-RD64 plants show a significant increase both in shoot and root fresh weight when compared to P-starved Mt-1021 plants. We discuss how, in a rhizobium-legume model system, a balanced interplay of different factors linked to the bacterial IAA over-production rather than IAA production per se stimulates plant growth under stressful environmental conditions, and in particular, under P-starvation.
2010-05-20 | GSE21737 | GEO