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Expression data from mouse neonatal ovarian DMBA and BaP culture experiments

ABSTRACT: Mammalian females are born with a finite number of non-renewing primordial follicles, the majority of which remain in a quiescent state for many years. Due to their non-renewing nature, these “resting” oocytes are particularly vulnerable to xenobiotic insult, resulting in premature ovarian senescence and the formation of dysfunctional oocytes. In this study we characterised the mechanisms of ovotoxicity for the ovotoxic agent 7,12-dimethylbenz-[a]anthracene (DMBA), which targets immature follicles. Neonatal mouse ovaries (PND3-4) were cultured in the presence of DMBA (50nM) or BaP (1uM) for 96 hours to observe their effects on the ovarian transcriptome. This was done in the hopes of gaining a better understanding of the mechanisms underpinning xenobiotic induced pre-antral ovotoxicity.

ORGANISM(S): Mus musculus

PROVIDER: GSE29263 | GEO | 2011/05/13

SECONDARY ACCESSION(S): PRJNA140269

REPOSITORIES: GEO

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Expression data from mouse neonatal ovarian DMBA and BaP culture experiments

Project description:Mammalian females are born with a finite number of non-renewing primordial follicles, the majority of which remain in a quiescent state for many years. Due to their non-renewing nature, these “resting” oocytes are particularly vulnerable to xenobiotic insult, resulting in premature ovarian senescence and the formation of dysfunctional oocytes. In this study we characterised the mechanisms of ovotoxicity for the ovotoxic agent 7,12-dimethylbenz-[a]anthracene (DMBA), which targets immature follicles. Neonatal mouse ovaries (PND3-4) were cultured in the presence of DMBA (50nM) or BaP (1uM) for 96 hours to observe their effects on the ovarian transcriptome. This was done in the hopes of gaining a better understanding of the mechanisms underpinning xenobiotic induced pre-antral ovotoxicity. Ovaries from day 3-4 Swiss neonatal mice were cultured in DMBA or BaP containing media for 96 hours. The ovaries were then collected for RNA extraction and hybridization on an Illumina Sentrix Mouse ref-8 v2 Beadchip

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