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Differential expression of genes after treatment with DAMTC in human lung adenocarcinoma cells (A549)

ABSTRACT: The experiment was designed to identify the genes which get altered after the treatment with DAMTC in A549 cells. The A549 cells plated in T25 flask were treated with DAMTC at a concentration of 160 microgram/ml for 24 h.

ORGANISM(S): Homo sapiens

PROVIDER: GSE29698 | GEO | 2012/05/01

SECONDARY ACCESSION(S): PRJNA141123

REPOSITORIES: GEO

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Differential expression of genes after treatment with DAMTC in human lung adenocarcinoma cells (A549)

Project description:The experiment was designed to identify the genes which get altered after the treatment with DAMTC in A549 cells. The A549 cells plated in T25 flask were treated with DAMTC at a concentration of 160 microgram/ml for 24 h. Biological triplicates of 2 samples were used viz. control A549 cells (Control), A549 cells treated with DAMTC (160 micrograms/ml) for 24 h (Treated)

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miRNA expression of exosomes derived from lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP

Project description:To investigate the difference of miRNA expression in exosomes derived from A549 cells and its DDP-resistant cell strain A549/DDP, we have employed miRNA microarray expression to discover the difference expression of miRNAs in exosomes derived from A549 and A549/DDP. We conducted RT-qPCR to examine the expression levels of top differential expressed miRNAs, namely, miR-197-5p, miR-4443, miR-642a-3p, miR-27b-3p and miR-100-5p, confirming low variability between two methods. The A549/DDP was established from A549 in our laboratory, by exposing A549 to gradually increasing DDP concentrations, until the final concentration at 1Î¼g/ml. To avoid the influence of drug to the A549/DDP cells, they were cultured in a drug-free medium for at least two weeks before gene expression analysis. After being incubated for 48-72h, the culture medium of cells was harvested. Exosomes were isolated by ultracentrifugation. And miRNA expression of exosomes derived from A549 and A549/DDP was then analzyed.

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