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2.45 GHz Radiofrequency fields alter gene expression in cultured human cells

ABSTRACT: The biological effect of radiofrequency (RF) fields remains controversial. We address this issue by examining whether RF fields can cause changes in gene expression. We observed that 221 genes altered their expression after a 2-hour exposure. The number of affected genes increased to 759 after a 6-hour exposure. Functional classification of the affected genes reveals that apoptosis-related genes were among the up-regulated ones and the cell cycle genes among the down-regulated ones. We observed no significant increase in the expression of heat shock genes. These results indicate that the RF fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism. Keywords: gene expression SAGE

ORGANISM(S): Homo sapiens

PROVIDER: GSE3025 | GEO | 2005/07/30

SECONDARY ACCESSION(S): PRJNA93213

REPOSITORIES: GEO

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2.45 GHz Radiofrequency fields alter gene expression in cultured human cells

Project description:The biological effect of radiofrequency (RF) fields remains controversial. We address this issue by examining whether RF fields can cause changes in gene expression. We observed that 221 genes altered their expression after a 2-hour exposure. The number of affected genes increased to 759 after a 6-hour exposure. Functional classification of the affected genes reveals that apoptosis-related genes were among the up-regulated ones and the cell cycle genes among the down-regulated ones. We observed no significant increase in the expression of heat shock genes. These results indicate that the RF fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism. Keywords: gene expression SAGE We used the pulsed RF fields at a frequency of 2.45 GHz that is commonly used in telecommunication to expose cultured human HL-60 cells. We used the SAGE (serial analysis of gene expression) method to measure the RF effect on gene expression at the genome level.

2005-07-30 | E-GEOD-3025 | biostudies-arrayexpress

Analysis of gene expression in mouse brain regions after exposure to pulse-modulated 1.9 GHz radiofrequency fields

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Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz continuous-wave radiofrequency fields

Project description:This study assessed changes in gene expression in 7 mouse brain regions using Illumina Mouse WG-6 (v2) beadchips after exposure to 1.9 GHz continuous-wave radiofrequency field exposure for 4h/day for 5 days. High dose RF field exposure was whole body average SAR and brain average SAR were 1.36 W/kg and 0.19 W/kg, respectively. Total RNA isolated from the amygdala, caudate, cerebellum, hippocampus, hypothalamus and medial prefrontal cortex of 1.9 GHz continuous-wave radiofrequency field exposed mice and compared to that in sham (handled) exposed animals. An unhandled control group was also included in the study design. A total of 20 RNA samples (5 independent biological tissues for each of 4 treatment groups) were collected for each brain region. When analysis of gene expression was conducted within individual brain regions when controlling the false discovery rate (FDR), no differentially expressed genes were identified relative to the sham control. However, it must be noted that most fold changes among groups were observed to be less than 1.5 fold and this study had limited ability to detect such small changes. While some genes were differentially expressed without correction for multiple-comparisons testing, no consistent pattern of response was observed among different RF-exposure levels or among different RF-modulations. The current study provides the most comprehensive analysis of potential gene expression changes in the rodent brain in response to RF field exposure conducted to date. Within the exposure conditions and limitations of this study, no convincing evidence of consistent changes in gene expression was found in response to 1.9 GHz pulse-modulated RF field exposure.

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Project description:Transcriptomic data for 48 and 120 hpf zebrafish embryos developmentally exposed to 3.5 GHz of radiofrequency radiation.

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Homo sapiens

Project description:2.45 GHz Radiofrequency fields alter gene expression in cultured human cells

| PRJNA93213 | ENA

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