Genomics

Dataset Information

0

Cell-type based analysis of microRNA profiles in the mouse brain


ABSTRACT: MicroRNAs (miRNA) are implicated in brain development and function but the underlying mechanisms have been difficult to study in part due to cellular heterogeneity in neural circuits. To systematically analyze miRNA expression in neurons, we have established a miRNA tagging and affinity purification (miRAP) method that is targeted to cell types through the Cre-loxP binary system in mice. Our studies of the neocortex and cerebellum reveal the expression of a large fraction of known miRNAs with distinct profiles in glutamatergic and GABAergic neurons, and subtypes of GABAergic neurons. We further detected putative novel miRNAs, tissue or cell type-specific strand selection of miRNAs, and miRNA editing. Our method thus will facilitate a systematic analysis of miRNA expression and regulation in specific neuron types in the context of neuronal development, physiology, plasticity, pathology and disease models, and is generally applicable to other cell types and tissues.

ORGANISM(S): Mus musculus

PROVIDER: GSE30286 | GEO | 2011/06/29

SECONDARY ACCESSION(S): PRJNA143587

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-06-29 | E-GEOD-30286 | biostudies-arrayexpress
2015-06-17 | E-GEOD-63137 | biostudies-arrayexpress
2015-06-17 | GSE63137 | GEO
2010-12-01 | E-MTAB-452 | biostudies-arrayexpress
2021-02-12 | GSE153665 | GEO
2021-12-15 | GSE188528 | GEO
2024-12-03 | GSE262320 | GEO
2024-09-16 | GSE262322 | GEO
2024-06-14 | GSE262321 | GEO
2024-08-24 | GSE275097 | GEO