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Transcriptional analysis of temperature regulation in Yersinia pestis

ABSTRACT: Temperature is a key environmental factor for facultative pathogens during the host adaptation response. To assess the functional role of temperature in Yersinia pestis, a microarray study was conducted comparing the Δpgm (pigmentation-negative) R88 strain grown at 37°C or 30°C.

ORGANISM(S): Yersinia pestis

PROVIDER: GSE30333 | GEO | 2016/01/01

SECONDARY ACCESSION(S): PRJNA154807

REPOSITORIES: GEO

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Transcriptional analysis of temperature regulation in Yersinia pestis

Project description:Temperature is a key environmental factor for facultative pathogens during the host adaptation response. To assess the functional role of temperature in Yersinia pestis, a microarray study was conducted comparing the Îpgm (pigmentation-negative) R88 strain grown at 37Â°C or 30Â°C. Six independent RNA samples from 37Â°C cultures were paired with six independent RNA samples from 30Â°C cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.

2016-07-03 | E-GEOD-30333 | biostudies-arrayexpress

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Project description:Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of autoinducer 2 (AI-2) quorum sensing was investigated by comparing transcript profiles when luxS gene was knocked out. The luxS gene encodes S-ribosylhomocysteinase which can produce DPD, a precursor of AI-2. The strain M-bM-^HM-^Fpgm (pigmentation-negative) mutant R88 was called wild type. The M-bM-^HM-^Fpgm M-bM-^HM-^FluxS mutant was called control. Six independent RNA samples from R88 were paired with six independent RNA samples from M-bM-^HM-^Fpgm M-bM-^HM-^FluxS mutant cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.

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