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Dissecting primary (translation independent) from secondary (translation dependent) IFN-mediated differential gene expression

ABSTRACT: NIH-3T3 cells were pretreated for 15 min with either DMSO (mock) or cycloheximide followed by addition of either mock, 100 U/ml IFNalpha or 100 U/ml IFNgamma for 1h. During the last 30 min, 500 µM 4-thiouridine was added to cell culture medium. Total cellular RNA was isolated using Trizol reagent and nascent RNA was purified as described (Dölken et al. RNA 2008) . Three replicates of nascent RNA were analyzed by Affymetrix Mouse Gene ST 1.0 arrays

ORGANISM(S): Mus musculus

PROVIDER: GSE30457 | GEO | 2013/07/04

SECONDARY ACCESSION(S): PRJNA143545

REPOSITORIES: GEO

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Dissecting primary (translation independent) from secondary (translation dependent) IFN-mediated differential gene expression

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