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Transcriptional characterization of tomato roots response to iron deficiency

ABSTRACT: Iron (Fe) deficiency is a yield-limiting factor for a variety of field crops across the world and generally results from the interaction of limited soil Fe bioavailability and susceptible genotype cultivation. Tomato, a Strategy I, model plant for Fe deficiency, is an important economical crop. Tomato responses in order to improve Fe uptake are based on acidification of rhizosphere, reduction of Fe3+ to Fe2+ and transport of Fe2+ into the cells.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE31112 | GEO | 2012/03/22

SECONDARY ACCESSION(S): PRJNA144681

REPOSITORIES: GEO

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Transcriptional characterization of tomato roots response to iron deficiency

Project description:Iron (Fe) deficiency is a yield-limiting factor for a variety of field crops across the world and generally results from the interaction of limited soil Fe bioavailability and susceptible genotype cultivation. Tomato, a Strategy I, model plant for Fe deficiency, is an important economical crop. Tomato responses in order to improve Fe uptake are based on acidification of rhizosphere, reduction of Fe3+ to Fe2+ and transport of Fe2+ into the cells. Transcriptional profile obtained by roots (27-d) of 21-d-old tomato plants starved of iron for an additional week was compared with the transcriptional profile obtained for roots (27-d) of 21-d-old tomato plants grown for an additional week at 100 M-NM-<M Fe. Tomato plants were hydroponically grown in both cases. Three different biological replicates were used for each sample repeating the experiment three times. All samples were obtained pooling roots of six plants (27-d-old).

2012-03-22 | E-GEOD-31112 | biostudies-arrayexpress

Root transcriptional profile of tomato Fe-deficient plants in response to supply with natural chelating agents.

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Transcriptome analysis reveals new insight into iron homeostasis in Populus tomentosa

Project description:Iron (Fe) is an essential micronutrient for the survival and proliferation of plants. Plants have evolved complex mechanisms to maintain Fe homeostasis in response of Fe deficiency conditions. To explore the mechanisms of Populus tomentosa response to Fe deficiency, we evaluated the physiological, biochemical and transcriptome differences of P. tomentosa between Fe-sufficient and Fe-deficient conditions. The results showed that, under Fe-free conditions, the chlorophyll synthesis and photosynthesis pathways in shoots were extremely depressed. The inhibition of these pathways caused chlorosis and reduced shoot growth. Meanwhile, although both two photosynthetic systems (PSI and PSII) were inhibited under Fe limited conditions, PSI is affected more serious and earlier than PSII. In order to maintain Fe homeostasis, several genes involved in Fe regulation network were differentially expressed. At the late period of Fe deficiency response, some genes (BTS, bHLH38/39 and PYE) in PYE regulatory network were up-regulated in roots, while some root-specific ethylene-dependent FIT regulatory genes (EIN3, ERF and FIT) were down-regulated. Moreover, FRO2 was induced in P. tomentosa roots to reduce more Fe3+, which is similar with other strategy I plants. It is interest that we found another Fe2+ transporter gene (NRAMP1) was induced, instead of the well-known Fe2+ transporter gene (IRT1) for strategy I plants, to promote Fe2+ absorption at the Fe deficiency late stage.

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Root transcriptional profile of tomato Fe-deficient plants in response to supply with natural chelating agents.

Project description:Tomato, a Strategy I model plant for Fe deficiency, is an important economical crop. The transcriptional responses induced by Fe deficiency in tomato roots were previously described (Zamboni et al., 2012). The changes in trascriptome caused by the supply of Fe to plants starved fro 1 week were described in relation to the different nature of chelating agents (Fe-WEHS, Fe-CITRATE and Fe-PS). Transcriptional profile obtained by roots (27-d) of 21-d-old tomato plants starved of iron (0 Î¼M Fe-EDTA) for 1 week and supplied for 1 h with 1 Î¼M of Fe as Fe-WEHS (supply_Fe_WEHS), Fe citrate (supply_Fe_CITRATE) and Fe-PS (supply_Fe_PS). Tomato plants were hydroponically grown in all three case of Fe supply. Three different biological replicates were used for each sample repeating the experiment three times. All samples were obtained pooling roots of six plants (27-d-old).

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Transcriptomic changes in maize roots in response to Fe deficiency

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